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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/10/2019 |
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Data da última atualização: |
12/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; FERNANDA QUEIROS COSTA, UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO; MICHELE MUNK, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; SABINE WOHLRES-VIANA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; RAQUEL VARELA SERAPIÃO, PESAGRO; BRUNO CAMPOS DE CARVALHO, CNPGL; PAULO HENRIQUE CAMPOS JÚNIOR, UFSJ; ALEX CABRAL VIEIRA, UFV; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF; JOAO HENRIQUE MOREIRA VIANA, CENARGEN. |
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Título: |
Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 10, p. 1357-1365, 2019. |
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DOI: |
https://doi.org/10.1111/rda.13544 |
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Idioma: |
Inglês |
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Conteúdo: |
This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes. MenosThis study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and ... Mostrar Tudo |
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Palavras-Chave: |
Activation; Aquaporin 3; Cleavage; Embryos; Heat Shock; Hsp70 Protein; Ionomycin; Maturation. |
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Thesaurus Nal: |
Apoptosis; Blastocyst; Gametocytes; Gene expression; In vitro fertilization; Oocytes; Parthenogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208435/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
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Marc: |
LEADER 02828naa a2200421 a 4500
001 2118469
005 2024-04-12
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/rda.13544$2DOI
100 1 $aCAMARGO, L. S. A.
245 $aContrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos.$h[electronic resource]
260 $c2019
520 $aThis study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes.
650 $aApoptosis
650 $aBlastocyst
650 $aGametocytes
650 $aGene expression
650 $aIn vitro fertilization
650 $aOocytes
650 $aParthenogenesis
653 $aActivation
653 $aAquaporin 3
653 $aCleavage
653 $aEmbryos
653 $aHeat Shock
653 $aHsp70 Protein
653 $aIonomycin
653 $aMaturation
700 1 $aCOSTA, F. Q.
700 1 $aMUNK, M.
700 1 $aWOHLRES-VIANA, S.
700 1 $aSERAPIÃO, R. V.
700 1 $aCARVALHO, B. C.
700 1 $aCAMPOS JÚNIOR, P. H.
700 1 $aVIEIRA, A. C.
700 1 $aNOGUEIRA, L. A. G.
700 1 $aVIANA, J. H. M.
773 $tReproduction in Domestic Animals$gv. 54, n. 10, p. 1357-1365, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
21/05/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CASARI, R. A. C. N.; PAIVA, D. S.; SILVA, V. N. B.; FERREIRA, T. M. M.; SOUZA JUNIOR, M. T.; OLIVEIRA, N. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SANTOS, T. T.; GOMIDE, R. L.; MAGALHÃES, P. C.; SOUSA, C. A. F. de. |
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Afiliação: |
Raphael Augusto das Chagas Noqueli Casari, Bolsista da Embrapa Agroenergia; Dayane S. Paiva, Bolsista da Embrapa Agroenergia; Vivianny N. B. Silva, Bolsista da Embrapa Agroenergia; Thalita M. M. Ferreira, Bolsista da Embrapa Agroenergia; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; Nelson G. Oliveira, Bolsista da Embrapa Agroenergia; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Using thermography to confirm genotypic variation for drought response in maize. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
International Journal of Molecular Sciences v. 20, n. 9, 2273, May-1, 2019. |
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DOI: |
10.3390/ijms20092273 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
The feasibility of thermography as a technique for plant screening aiming at drought-tolerance has been proven by its relationship with gas exchange, biomass, and yield. In this study, unlike most of the previous, thermography was applied for phenotyping contrasting maize genotypes whose classification for drought tolerance had already been established in the field. Our objective was to determine whether thermography-based classification would discriminate the maize genotypes in a similar way as the field selection in which just grain yield was taken into account as a criterion. We evaluated gas exchange, daily water consumption, leaf relative water content, aboveground biomass, and grain yield. Indeed, the screening of maize genotypes based on canopy temperature showed similar results to traditional methods. Nevertheless, canopy temperature only partially reflected gas exchange rates and daily water consumption in plants under drought. Part of the explanation may lie in the changes that drought had caused in plant leaves and canopy structure, altering absorption and dissipation of energy, photosynthesis, transpiration, and partitioning rates. Accordingly, although there was a negative relationship between grain yield and plant canopy temperature, it does not necessarily mean that plants whose canopies were maintained cooler under drought achieved the highest yield. |
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Palavras-Chave: |
Canopy temperature; Déficit hídrico; Fenotipagem de plantas; Plant phenotyping; Termografia; Thermal image; Tolerância à seca; Water deficit. |
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Thesagro: |
Milho; Zea Mays. |
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Thesaurus NAL: |
Abiotic stress; Drought tolerance; Thermography. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198719/1/SEG-05.12.12.001.00.05-HUGO-3-ijms-20-02273-v2.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197552/1/Artigo-termografia.pdf
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Marc: |
LEADER 02607naa a2200421 a 4500
001 2109974
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3390/ijms20092273$2DOI
100 1 $aCASARI, R. A. C. N.
245 $aUsing thermography to confirm genotypic variation for drought response in maize.$h[electronic resource]
260 $c2019
520 $aThe feasibility of thermography as a technique for plant screening aiming at drought-tolerance has been proven by its relationship with gas exchange, biomass, and yield. In this study, unlike most of the previous, thermography was applied for phenotyping contrasting maize genotypes whose classification for drought tolerance had already been established in the field. Our objective was to determine whether thermography-based classification would discriminate the maize genotypes in a similar way as the field selection in which just grain yield was taken into account as a criterion. We evaluated gas exchange, daily water consumption, leaf relative water content, aboveground biomass, and grain yield. Indeed, the screening of maize genotypes based on canopy temperature showed similar results to traditional methods. Nevertheless, canopy temperature only partially reflected gas exchange rates and daily water consumption in plants under drought. Part of the explanation may lie in the changes that drought had caused in plant leaves and canopy structure, altering absorption and dissipation of energy, photosynthesis, transpiration, and partitioning rates. Accordingly, although there was a negative relationship between grain yield and plant canopy temperature, it does not necessarily mean that plants whose canopies were maintained cooler under drought achieved the highest yield.
650 $aAbiotic stress
650 $aDrought tolerance
650 $aThermography
650 $aMilho
650 $aZea Mays
653 $aCanopy temperature
653 $aDéficit hídrico
653 $aFenotipagem de plantas
653 $aPlant phenotyping
653 $aTermografia
653 $aThermal image
653 $aTolerância à seca
653 $aWater deficit
700 1 $aPAIVA, D. S.
700 1 $aSILVA, V. N. B.
700 1 $aFERREIRA, T. M. M.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aOLIVEIRA, N. G.
700 1 $aKOBAYASHI, A. K.
700 1 $aMOLINARI, H. B. C.
700 1 $aSANTOS, T. T.
700 1 $aGOMIDE, R. L.
700 1 $aMAGALHÃES, P. C.
700 1 $aSOUSA, C. A. F. de
773 $tInternational Journal of Molecular Sciences$gv. 20, n. 9, 2273, May-1, 2019.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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