Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
16/02/2016 |
Data da última atualização: |
16/02/2016 |
Autoria: |
MARCONI, E. C. M.; BERNARDES, N. T. C. G.; BESERRA, L. A. R.; SILVA, F. D. F.; GREGORI, F. |
Afiliação: |
ELIZABETH C. M. MARCONI, USP; NARA T. C. G. BERNARDES, USP; LAILA A. R. BESERRA, USP; FERNANDA D. F. SILVA, USP; FABIO GREGORI, USP. |
Título: |
Development of a Real-time PCR for porcine group A rotavirus diagnosis. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n.1, p. 39-43, jan. 2015. |
Idioma: |
Inglês |
Conteúdo: |
oped to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR and genetic sequencing). The overall agreement (kappa) was 0.843, indicating ?very good? concordance between tests, presenting 100% of relative sensitivity (25+ Real Time PCR/25+ Conventional PCR) and 87.5% of relative sensitivity (35- Real Time PCR/40- Conventional PCR). The results also demonstrated high intra- and inter-assay reproducibility (coefficient of variation ≤1.42%); thus, this method proved to be a fast and sensitive approach for the diagnosis of RVA in pigs. |
Palavras-Chave: |
PCR; PCR em tempo real. |
Thesagro: |
Diagnóstico; Rotavírus; Suíno. |
Thesaurus Nal: |
Quantitative polymerase chain reaction; Swine. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139159/1/Development-of-a-real-time.pdf
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Marc: |
LEADER 01483naa a2200253 a 4500 001 2037231 005 2016-02-16 008 2015 bl uuuu u00u1 u #d 100 1 $aMARCONI, E. C. M. 245 $aDevelopment of a Real-time PCR for porcine group A rotavirus diagnosis. 260 $c2015 520 $aoped to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR and genetic sequencing). The overall agreement (kappa) was 0.843, indicating ?very good? concordance between tests, presenting 100% of relative sensitivity (25+ Real Time PCR/25+ Conventional PCR) and 87.5% of relative sensitivity (35- Real Time PCR/40- Conventional PCR). The results also demonstrated high intra- and inter-assay reproducibility (coefficient of variation ≤1.42%); thus, this method proved to be a fast and sensitive approach for the diagnosis of RVA in pigs. 650 $aQuantitative polymerase chain reaction 650 $aSwine 650 $aDiagnóstico 650 $aRotavírus 650 $aSuíno 653 $aPCR 653 $aPCR em tempo real 700 1 $aBERNARDES, N. T. C. G. 700 1 $aBESERRA, L. A. R. 700 1 $aSILVA, F. D. F. 700 1 $aGREGORI, F. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n.1, p. 39-43, jan. 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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