01483naa a2200253 a 450000100080000000500110000800800410001910000220006024500760008226000090015852007650016765000430093265000100097565000170098565000150100265000110101765300080102865300220103670000270105870000220108570000200110770000160112777300860114320372312016-02-16       2015    bl uuuu     u00u1 u    #d1 aMARCONI, E. C. M.  aDevelopment of a Real-time PCR for porcine group A rotavirus diagnosis.  c2015  aoped to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR and genetic sequencing). The overall agreement (kappa) was 0.843, indicating ?very good? concordance between tests, presenting 100% of relative sensitivity (25+ Real Time PCR/25+ Conventional PCR) and 87.5% of relative sensitivity (35- Real Time PCR/40- Conventional PCR). The results also demonstrated high intra- and inter-assay reproducibility (coefficient of variation ≤1.42%); thus, this method proved to be a fast and sensitive approach for the diagnosis of RVA in pigs.  aQuantitative polymerase chain reaction  aSwine  aDiagnóstico  aRotavírus  aSuíno  aPCR  aPCR em tempo real1 aBERNARDES, N. T. C. G.1 aBESERRA, L. A. R.1 aSILVA, F. D. F.1 aGREGORI, F.  tPesquisa Veterinária Brasileira, Brasília, DFgv. 35, n.1, p. 39-43, jan. 2015.