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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
29/04/2016 |
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Data da última atualização: |
29/04/2016 |
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Autoria: |
SPRADA, A. G.; ROSA, M. P. da; MACHADO, A. K.; PIPPI, N. L.; BAYARD, P.; CRUZ, I. B. M. da. |
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Afiliação: |
ARÍCIA GOMES SPRADA, CCR/UFSM; MATHEUS PIPPI DA ROSA, CCR/UFSM; ALENCAR KOLINSKI MACHADO, CCS/UFSM; NEY LUIS PIPPI, CCR/UFSM; PAULO BAYARD, CCR/UFSM; IVANA BEATRICE MÂNICA DA CRUZ, CCS/UFSM. |
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Título: |
Toxicity and oxidative stress of canine mesanchymal stromal cells from adipose tissue in different culture passages. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, supl. 1, p. 15-20, dez. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Stem cells in regenerative therapy have received attention from researchers in recent decades. The culture of these cells allows studies about their behavior and metabolism. Thus, cell culture is the basis for cell therapy and tissue engineering researches. A major concern regarding the use of cultivated stem cell in human or veterinary clinical routine is the risk of carcinogenesis. Cellular activities require a balanced redox state. However, when there is an imbalance in this state, oxidative stress occurs. Oxidative stress contributes to cytotoxicity, which may result in cell death or genomic alterations, favoring the development of cancer cells. The aim of this study was to determine whether there are differences in the behavior of cultured mesenchymal stem cells from canine adipose tissue according to its site of collection (omentum and subcutaneous) evaluating the rate of proliferation, viability, level of oxidative stress and cytotoxicity over six passages. For this experiment, two samples of adipose tissue from subcutaneous and omentum where taken from a female dog corpse, 13 years old, Pitbull. The results showed greater levels of oxidative stress in the first and last passages of both groups, favoring cytotoxicity and cell death. |
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Palavras-Chave: |
Células-tronco; Culture; Passage; Passagem; Radicais livres; Stem cell; Viabilidade celular. |
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Thesagro: |
Produção vegetal. |
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Thesaurus Nal: |
Cell viability; Free radicals. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142669/1/Toxicity-and-oxidative-stress.pdf
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Marc: |
LEADER 02131naa a2200301 a 4500
001 2044244
005 2016-04-29
008 2015 bl uuuu u00u1 u #d
100 1 $aSPRADA, A. G.
245 $aToxicity and oxidative stress of canine mesanchymal stromal cells from adipose tissue in different culture passages.
260 $c2015
520 $aStem cells in regenerative therapy have received attention from researchers in recent decades. The culture of these cells allows studies about their behavior and metabolism. Thus, cell culture is the basis for cell therapy and tissue engineering researches. A major concern regarding the use of cultivated stem cell in human or veterinary clinical routine is the risk of carcinogenesis. Cellular activities require a balanced redox state. However, when there is an imbalance in this state, oxidative stress occurs. Oxidative stress contributes to cytotoxicity, which may result in cell death or genomic alterations, favoring the development of cancer cells. The aim of this study was to determine whether there are differences in the behavior of cultured mesenchymal stem cells from canine adipose tissue according to its site of collection (omentum and subcutaneous) evaluating the rate of proliferation, viability, level of oxidative stress and cytotoxicity over six passages. For this experiment, two samples of adipose tissue from subcutaneous and omentum where taken from a female dog corpse, 13 years old, Pitbull. The results showed greater levels of oxidative stress in the first and last passages of both groups, favoring cytotoxicity and cell death.
650 $aCell viability
650 $aFree radicals
650 $aProdução vegetal
653 $aCélulas-tronco
653 $aCulture
653 $aPassage
653 $aPassagem
653 $aRadicais livres
653 $aStem cell
653 $aViabilidade celular
700 1 $aROSA, M. P. da
700 1 $aMACHADO, A. K.
700 1 $aPIPPI, N. L.
700 1 $aBAYARD, P.
700 1 $aCRUZ, I. B. M. da
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, supl. 1, p. 15-20, dez. 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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Data corrente: |
14/08/2020 |
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Data da última atualização: |
10/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LIMA, A. O. de; KOLTES, J. E.; DINIZ, W. J. S.; OLIVEIRA, P. S. N. de; CESAR, A. S. M.; TIZIOTO, P. L.; AFONSO, J.; SOUZA, M. de S.; PETRINI, J.; ROCHA, M. I. P.; CARDOSO, T. F.; ZERLOTINI NETO, A.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A. |
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Afiliação: |
ANDRESSA O. DE LIMA, UFSCar; JAMES E. KOLTES, Iowa State University; WELLISON J. S. DINIZ, UFSCar; PRISCILA S. N. DE OLIVEIRA; ALINE S. M. CESAR, Esalq/USP; POLYANA C. TIZIOTO, NGS Genomic Solution; JULIANA AFONSO, UFSCar; MARCELA M. DE SOUZA, Iowa State University; JULIANA PETRINI, UNIFAL; MARINA I. P. ROCHA, UFSCar; TAINÃ F. CARDOSO; ADHEMAR ZERLOTINI NETO, CNPTIA; LUIZ L. COUTINHO, Esalq/USP; GERSON B. MOURÃO, Esalq/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Potential biomarkers for feed efficiency-related traits in nelore cattle identified by co-expression network and integrative genomics analyses. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Genetics, v. 11, p. 1-14, Mar. 2020. |
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DOI: |
https://doi.org/10.3389/fgene.2020.00189 |
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Idioma: |
Inglês |
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Notas: |
Article 189. Na publicação: Luciana C. A. Regitano. |
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Conteúdo: |
Feed efficiency helps to reduce environmental impacts from livestock production, improving beef cattle profitability. We identified potential biomarkers (hub genes) for feed efficiency, by applying co-expression analysis in Longissimus thoracis RNA-Seq data from 180 Nelore steers. Six co-expression modules were associated with six feed efficiency-related traits (p-value ≤ 0.05). Within these modules, 391 hub genes were enriched for pathways as protein synthesis, muscle growth, and immune response. Trait-associated transcription factors (TFs) ELF1, ELK3, ETS1, FLI1, and TCF4, were identified with binding sites in at least one hub gene. Gene expression of CCDC80, FBLN5, SERPINF1, and OGN was associated with multiple feed efficiency-related traits (FDR ≤ 0.05) and were previously related to glucose homeostasis, oxidative stress, fat mass, and osteoblastogenesis, respectively. Potential regulatory elements were identified, integrating the hub genes with previous studies from our research group, such as the putative cis-regulatory elements (eQTLs) inferred as affecting the PCDH18 and SPARCL1 hub genes related to immune system and adipogenesis, respectively. Therefore, our analyses contribute to a better understanding of the biological mechanisms underlying feed efficiency in bovine and the hub genes disclosed can be used as biomarkers for feed efficiency-related traits in Nelore cattle. |
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Palavras-Chave: |
Biomarcadores; Expressão gênica; Feed efficiency; Hub genes; Longissimus thoracis; Systems biology; WGCNA. |
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Thesagro: |
Bos Indicus. |
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Thesaurus NAL: |
Gene expression. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215312/1/AP-Potential-biomarkers-2020.pdf
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Marc: |
LEADER 02711naa a2200421 a 4500
001 2124356
005 2020-09-10
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fgene.2020.00189$2DOI
100 1 $aLIMA, A. O. de
245 $aPotential biomarkers for feed efficiency-related traits in nelore cattle identified by co-expression network and integrative genomics analyses.$h[electronic resource]
260 $c2020
500 $aArticle 189. Na publicação: Luciana C. A. Regitano.
520 $aFeed efficiency helps to reduce environmental impacts from livestock production, improving beef cattle profitability. We identified potential biomarkers (hub genes) for feed efficiency, by applying co-expression analysis in Longissimus thoracis RNA-Seq data from 180 Nelore steers. Six co-expression modules were associated with six feed efficiency-related traits (p-value ≤ 0.05). Within these modules, 391 hub genes were enriched for pathways as protein synthesis, muscle growth, and immune response. Trait-associated transcription factors (TFs) ELF1, ELK3, ETS1, FLI1, and TCF4, were identified with binding sites in at least one hub gene. Gene expression of CCDC80, FBLN5, SERPINF1, and OGN was associated with multiple feed efficiency-related traits (FDR ≤ 0.05) and were previously related to glucose homeostasis, oxidative stress, fat mass, and osteoblastogenesis, respectively. Potential regulatory elements were identified, integrating the hub genes with previous studies from our research group, such as the putative cis-regulatory elements (eQTLs) inferred as affecting the PCDH18 and SPARCL1 hub genes related to immune system and adipogenesis, respectively. Therefore, our analyses contribute to a better understanding of the biological mechanisms underlying feed efficiency in bovine and the hub genes disclosed can be used as biomarkers for feed efficiency-related traits in Nelore cattle.
650 $aGene expression
650 $aBos Indicus
653 $aBiomarcadores
653 $aExpressão gênica
653 $aFeed efficiency
653 $aHub genes
653 $aLongissimus thoracis
653 $aSystems biology
653 $aWGCNA
700 1 $aKOLTES, J. E.
700 1 $aDINIZ, W. J. S.
700 1 $aOLIVEIRA, P. S. N. de
700 1 $aCESAR, A. S. M.
700 1 $aTIZIOTO, P. L.
700 1 $aAFONSO, J.
700 1 $aSOUZA, M. de S.
700 1 $aPETRINI, J.
700 1 $aROCHA, M. I. P.
700 1 $aCARDOSO, T. F.
700 1 $aZERLOTINI NETO, A.
700 1 $aCOUTINHO, L. L.
700 1 $aMOURÃO, G. B.
700 1 $aREGITANO, L. C. de A.
773 $tFrontiers in Genetics$gv. 11, p. 1-14, Mar. 2020.
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Embrapa Agricultura Digital (CNPTIA) |
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