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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
29/11/2022 |
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Data da última atualização: |
29/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAVALCANTE, M. de A.; OLIVEIRA, J. dos S.; BARRETO, M. S. da S.; PINHEIRO, L. P.; CANTUÁRIA, P. de C.; BORGES, W. L.; SILVA, G. A. da; SOUZA, T. M. de. |
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Afiliação: |
MARÍLIA DE A. CAVALCANTE, INSTITUTO FEDERAL DO AMAPÁ; JANYNA DOS S. OLIVEIRA, INSTITUTO FEDERAL DO AMAPÁ; MAYRA S. DA S. BARRETO, UNIVERSIDADE DO ESTADO DO AMAPÁ; LUCAS P. PINHEIRO, UNIVERSIDADE DO ESTADO DO AMAPÁ; PATRICK DE C. CANTUÁRIA, INSTITUTO DE PESQUISAS CIENTÍFICAS E TECNOLÓGICAS DO ESTADO DO AMAPÁ; WARDSSON LUSTRINO BORGES, CPAF-AP; GABRIEL A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAPÁ; TIAGO MARCOLINO DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAPÁ. |
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Título: |
An HPLC method to determine phenolic compounds of plant extracts: application to Byrsonima crassifolia and Senna alata leaves. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pharmacognosy Research, v. 14, n. 4, p. 395-404, 2022. |
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DOI: |
10.5530/pres.14.4.58 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. MenosBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the... Mostrar Tudo |
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Palavras-Chave: |
Compostos bioativos; Método cromatográfico. |
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Thesagro: |
Cromatografia; Extrato Vegetal. |
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Thesaurus Nal: |
Bioactive compounds; Plant extracts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148941/1/CPAF-AP-AnHPLCMethod.pdf
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Marc: |
LEADER 02625naa a2200289 a 4500
001 2148941
005 2022-11-29
008 2022 bl uuuu u00u1 u #d
024 7 $a10.5530/pres.14.4.58$2DOI
100 1 $aCAVALCANTE, M. de A.
245 $aAn HPLC method to determine phenolic compounds of plant extracts$bapplication to Byrsonima crassifolia and Senna alata leaves.$h[electronic resource]
260 $c2022
520 $aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.
650 $aBioactive compounds
650 $aPlant extracts
650 $aCromatografia
650 $aExtrato Vegetal
653 $aCompostos bioativos
653 $aMétodo cromatográfico
700 1 $aOLIVEIRA, J. dos S.
700 1 $aBARRETO, M. S. da S.
700 1 $aPINHEIRO, L. P.
700 1 $aCANTUÁRIA, P. de C.
700 1 $aBORGES, W. L.
700 1 $aSILVA, G. A. da
700 1 $aSOUZA, T. M. de
773 $tPharmacognosy Research$gv. 14, n. 4, p. 395-404, 2022.
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
17/09/2007 |
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Data da última atualização: |
29/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - C |
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Autoria: |
LEITE, K. C. E.; COLLEVATTI, R. G.; MENEGASSO, T. R.; TOMAS, W. M.; DUARTE, J. M. L. |
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Afiliação: |
K. C. E. Leite, Universidade Católica de Brasília; R. G. Collevatti, Universidade Católica de Brasília; T. R. Menegasso, Universidade Estadual Paulista; Walfrido Moraes Tomas, Embrapa Pantanal; J. M. B. Duarte, Universidade Estadual Paulista. |
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Título: |
Transferability of microsatellite loci from Cervidae species to the endangered Brazilian marsh deer, Blastocerus dichotomus. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.6, n.2, p.325-330, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Blastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroeletric power station construction and agricultural land expansion. In the present study we tested 38 microsatellite loci from four Cervidae species: Odocoileus virginianus (7), Rangifer tarandus (17), Capreolus capreolus (7), and Mazama bororo (7). Eleven loci showed clear amplification, opening a new perspective for the generation of fundamental population genetic data dor devising conservation strategies for B. dichtomus. |
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Thesagro: |
Blastocerus Dichotomus. |
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Thesaurus NAL: |
Cervidae. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171785/1/Tomas.pdf
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Marc: |
LEADER 01255naa a2200193 a 4500
001 1812435
005 2018-01-29
008 2007 bl uuuu u00u1 u #d
100 1 $aLEITE, K. C. E.
245 $aTransferability of microsatellite loci from Cervidae species to the endangered Brazilian marsh deer, Blastocerus dichotomus.$h[electronic resource]
260 $c2007
520 $aBlastocerus dichotomus, the marsh deer, is the largest Brazilian Cervidae species. The species is endangered because of hunting and loss of its natural habitat, i.e., flood plain areas, because of hydroeletric power station construction and agricultural land expansion. In the present study we tested 38 microsatellite loci from four Cervidae species: Odocoileus virginianus (7), Rangifer tarandus (17), Capreolus capreolus (7), and Mazama bororo (7). Eleven loci showed clear amplification, opening a new perspective for the generation of fundamental population genetic data dor devising conservation strategies for B. dichtomus.
650 $aCervidae
650 $aBlastocerus Dichotomus
700 1 $aCOLLEVATTI, R. G.
700 1 $aMENEGASSO, T. R.
700 1 $aTOMAS, W. M.
700 1 $aDUARTE, J. M. L.
773 $tGenetics and Molecular Research$gv.6, n.2, p.325-330, 2007.
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Registro original: |
Embrapa Pantanal (CPAP) |
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