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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
07/10/2020 |
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Data da última atualização: |
09/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MALOSSI, C. D.; FIORATTI, E. G.; CARDOSO, J. F.; MAGRO, A. J.; KROON, E. G.; AGUIAR, D. M. de; BORGES, A. M. C. M; NOGUEIRA, M. F.; ULLMANN, L. S.; ARAUJO JUNIOR, J. P. |
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Afiliação: |
CAMILA DANTAS MALOSSI, São Paulo State University (Unesp), Botucatu; EDUARDO GORZONI FIORATTI, Vales do Jequitinhonha e Mucuri Federal University (UFVJM); JEDSON FERREIRA CARDOSO, Evandro Chagas Institute, Ananindeua; ANGELO JOSE MAGRO, São Paulo State University, Unesp, Botucatu; ERNA GEESSIEN KROON, Universidade Federal de Minas Gerais; DANIEL MOURA DE AGUIAR, Mato Grosso Federal University, Cuiabá; ALICE MAMEDE COSTA MARQUE BORGES, Mato Grosso Federal University, Cuiabá; MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP; LEILA SABRINA ULLMANN, São Paulo State University, Unesp; JOÃO PESSOA ARAUJO JUNIOR, São Paulo State University, Unesp. |
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Título: |
High genomic variability in Equine Infectious Anemia Virus obtained from naturally infected horses in Pantanal, Brazil: an endemic region case. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Viruses, v. 12, n. 2, 207, p. 1-15, 2020. |
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DOI: |
10.3390/v12020207 |
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Idioma: |
Português |
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Conteúdo: |
Equine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions. MenosEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. Th... Mostrar Tudo |
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Thesagro: |
Anemia Infecciosa; Doença Animal; Eqüino; Vírus. |
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Thesaurus Nal: |
Endemic diseases; Equine infectious anemia virus. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216482/1/HighGenomicVariability-2020.pdf
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Marc: |
LEADER 02584naa a2200313 a 4500
001 2125345
005 2020-10-09
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3390/v12020207$2DOI
100 1 $aMALOSSI, C. D.
245 $aHigh genomic variability in Equine Infectious Anemia Virus obtained from naturally infected horses in Pantanal, Brazil$ban endemic region case.$h[electronic resource]
260 $c2020
520 $aEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions.
650 $aEndemic diseases
650 $aEquine infectious anemia virus
650 $aAnemia Infecciosa
650 $aDoença Animal
650 $aEqüino
650 $aVírus
700 1 $aFIORATTI, E. G.
700 1 $aCARDOSO, J. F.
700 1 $aMAGRO, A. J.
700 1 $aKROON, E. G.
700 1 $aAGUIAR, D. M. de
700 1 $aBORGES, A. M. C. M
700 1 $aNOGUEIRA, M. F.
700 1 $aULLMANN, L. S.
700 1 $aARAUJO JUNIOR, J. P.
773 $tViruses$gv. 12, n. 2, 207, p. 1-15, 2020.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Biblioteca |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Clima Temperado. |
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Data corrente: |
31/01/2024 |
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Data da última atualização: |
31/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
POERSCH, A. H.; STRECK, N. A.; HEINEMANN, A. B.; STEINMETZ, S.; ZANON, A. J.; SILVA, M. R. da; ROSSATO, I. G. |
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Afiliação: |
ANDERSON H. POERSCH, UNIVERSIDADE FEDERAL DE SANTA MARIA; NEREU A. STRECK, UNIVERSIDADE FEDERAL DE SANTA MARIA; ALEXANDRE BRYAN HEINEMANN, CNPAF; SILVIO STEINMETZ, CPACT; ALENCAR J. ZANON, UNIVERSIDADE FEDERAL DE SANTA MARIA; MICHEL R. DA SILVA, CROPS TEAM, Santa Maria-RS; IORAN G. ROSSATO, UNIVERSIDADE FEDERAL DE SANTA MARIA. |
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Título: |
Calibration and evaluation of new irrigated rice cultivars in the SimulArroz model. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Revista Brasileira de Engenharia Agrícola e Ambiental, v. 28, n. 5, e272761, maio 2024. |
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ISSN: |
1807-1929 |
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DOI: |
https://doi.org/10.1590/1807-1929/agriambi.v28n5e272761 |
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Idioma: |
Inglês |
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Conteúdo: |
Given genetical coefficients need to be calibrated for the most important cultivars on the market, new cultivars must be added to models such as SimulArroz. Thus, the aim of this study was to calibrate and evaluate the SimulArroz model for two new irrigated rice cultivars. The experiments were conducted in the municipality of Goianira in Goiás state during four growing seasons (2014/15, 2015/16, 2016/17, 2017/18) and in Rio Grande do Sul state in the municipalities of Alegrete (2015/16), Cachoeirinha (2015/ 16), Capão do Leão (2016/17, 2017/18), Santa Vitória do Palmar (2017/18) and Uruguaiana (2014/15, 2015/16). A randomized block design was used, with four replicates in Rio Grande do Sul and sowing plots in Goianira. The BRS Catiana and BRS Pampa cultivars were used and the Haun stage (HS), phenology, shoot dry matter biomass and yield were evaluated. The root mean square error (RMSE) for above-ground dry matter ranged from 51.7 to 577 g m -2, and for yield, the normalized root mean square error (NRMSE) ranged from 24 to 32% and 22 to 35% for the potential and high technological levels, respectively. The SimulArroz model was able to satisfactorily predict the growth, development, and yield of the BRS Catiana and BRS Pampa cultivars, increasing their area of application, including the tropical region of Brazil. |
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Palavras-Chave: |
Calibração; Coeficientes genéticos; SimulArroz. |
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Thesagro: |
Arroz Irrigado; Fenologia; Modelo de Simulação; Oryza Sativa; Variedade. |
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Thesaurus NAL: |
Calibration; Cultivars; Phenology; Rice; Simulation models. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161496/1/agriambi-2024.pdf
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Marc: |
LEADER 02425naa a2200373 a 4500
001 2161496
005 2024-01-31
008 2024 bl uuuu u00u1 u #d
022 $a1807-1929
024 7 $ahttps://doi.org/10.1590/1807-1929/agriambi.v28n5e272761$2DOI
100 1 $aPOERSCH, A. H.
245 $aCalibration and evaluation of new irrigated rice cultivars in the SimulArroz model.$h[electronic resource]
260 $c2024
520 $aGiven genetical coefficients need to be calibrated for the most important cultivars on the market, new cultivars must be added to models such as SimulArroz. Thus, the aim of this study was to calibrate and evaluate the SimulArroz model for two new irrigated rice cultivars. The experiments were conducted in the municipality of Goianira in Goiás state during four growing seasons (2014/15, 2015/16, 2016/17, 2017/18) and in Rio Grande do Sul state in the municipalities of Alegrete (2015/16), Cachoeirinha (2015/ 16), Capão do Leão (2016/17, 2017/18), Santa Vitória do Palmar (2017/18) and Uruguaiana (2014/15, 2015/16). A randomized block design was used, with four replicates in Rio Grande do Sul and sowing plots in Goianira. The BRS Catiana and BRS Pampa cultivars were used and the Haun stage (HS), phenology, shoot dry matter biomass and yield were evaluated. The root mean square error (RMSE) for above-ground dry matter ranged from 51.7 to 577 g m -2, and for yield, the normalized root mean square error (NRMSE) ranged from 24 to 32% and 22 to 35% for the potential and high technological levels, respectively. The SimulArroz model was able to satisfactorily predict the growth, development, and yield of the BRS Catiana and BRS Pampa cultivars, increasing their area of application, including the tropical region of Brazil.
650 $aCalibration
650 $aCultivars
650 $aPhenology
650 $aRice
650 $aSimulation models
650 $aArroz Irrigado
650 $aFenologia
650 $aModelo de Simulação
650 $aOryza Sativa
650 $aVariedade
653 $aCalibração
653 $aCoeficientes genéticos
653 $aSimulArroz
700 1 $aSTRECK, N. A.
700 1 $aHEINEMANN, A. B.
700 1 $aSTEINMETZ, S.
700 1 $aZANON, A. J.
700 1 $aSILVA, M. R. da
700 1 $aROSSATO, I. G.
773 $tRevista Brasileira de Engenharia Agrícola e Ambiental$gv. 28, n. 5, e272761, maio 2024.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Origem |
Tipo/Formato |
Classificação |
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