02584naa a2200313 a 450000100080000000500110000800800410001902400270006010000190008724501710010626000090027752016370028665000210192365000350194465000220197965000190200165000120202065000110203270000200204370000190206370000170208270000170209970000210211670000230213770000200216070000190218070000250219977300460222421253452020-10-09       2020    bl uuuu     u00u1 u    #d7 a10.3390/v120202072DOI1 aMALOSSI, C. D.  aHigh genomic variability in Equine Infectious Anemia Virus obtained from naturally infected horses in Pantanal, Brazilban endemic region case.h[electronic resource]  c2020  aEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectiousanemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory inthis area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAVare currently available only forgagand LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic areain Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing(NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning,Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilians train comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions.  aEndemic diseases  aEquine infectious anemia virus  aAnemia Infecciosa  aDoença Animal  aEqüino  aVírus1 aFIORATTI, E. G.1 aCARDOSO, J. F.1 aMAGRO, A. J.1 aKROON, E. G.1 aAGUIAR, D. M. de1 aBORGES, A. M. C. M1 aNOGUEIRA, M. F.1 aULLMANN, L. S.1 aARAUJO JUNIOR, J. P.  tVirusesgv. 12, n. 2, 207, p. 1-15, 2020.