01500naa a2200289 a 450000100080000000500110000800800410001902400580006010000170011824501570013526000090029252005600030165000130086165000300087465000110090465000290091565300130094465300180095765300280097565300280100370000200103170000240105170000250107570000190110070000210111977300700114019859152014-05-12       2014    bl uuuu     u00u1 u    #d7 aDOI http://dx.doi.org/10.4238/2014.February.21.82DOI1 aMUNIZ, C. R.  aAgrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.h[electronic resource]  c2014  aLasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.  agummosis  aAgrobacterium Tumefaciens  aGomose  aLasiodiplodia Theobromae  aCajueiro  aCashew plants  aMediated transformation  aTransformação mediada1 aSILVA, G. F. da1 aSOUZA JUNIOR, M. T.1 aFREIRE, F. das C. O.1 aKEMA, G. H. J.1 aGUEDES, M. I. F.  tGenetics and Molecular Researchgv. 13, n. 2, p. 2906-2913, 2014.