01666naa a2200253 a 450000100080000000500110000800800410001910000180006024500780007826000090015652010000016565000160116565000160118165000120119765000180120965000220122765000110124965000110126065300190127170000200129070000210131070000220133177300590135319851632023-05-25       2014    bl uuuu     u00u1 u    #d1 aDALTRO, C. B.  aGenetic diversity studies of Papaya meleira virus.h[electronic resource]  c2014  aPapaya (Carica papaya) is a fruit crop of great economic and social importance for Brazil and other papaya-producing countries. Brazil is the second largest producer in the world. The papaya sticky disease, caused by Papaya meleira virus (PMeV), has caused great losses in the major Brazilian papaya-producing states. In order to estimate the genetic diversity of PMeV, latex samples were collected from papaya plants in the states of Bahia, Espírito Santo, Pernambuco, Ceará and Rio Grande do Norte, and total RNA was extracted. Specific primer for the replicase region allowed the amplification, by RT-PCR, of a fragment of approximately 560 bp from 31 isolates. The sequence analysis indicated a level of conservation greater than 88% among isolates. Furthermore, comparative analyzes indicated that PMeV has similarity with mycoviruses of the family Totiviridae. This phylogenetic relationship was reinforced by the presence of conserved motifs within in the RdRp regions from mycoviruses.  aTotiviridae  aVariability  aViruses  aCarica Papaya  aDoença de planta  aMamão  aVírus  aSticky disease1 aABREU, E. F. M.1 aARAGAO, F. J. L.1 aANDRADE, E. C. de  tTropical Plant Pathologygv. 39, n.1, p.104-108, 2014.