02353nam a2200157 a 450000100080000000500110000800800410001910000170006024501220007726001400019952017930033965000180213265000170215065300100216770000180217719836112025-08-06       2013    bl uuuu     u00u1 u    #d1 aPAULA, D. P.  aMolecular approaches in biological controlbevaluating trophic interactions in agroecosystems.h[electronic resource]  aIn: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapac2013  aMolecular techniques provide valuable opportunities to study complex trophic interactions in the field. The main tools so far applied include radioisotope labeling, electrophoretic detection of prey isoenzymes, the detection of prey pigments by chromatographic analysis, a range of immunological approaches to detect prey proteins (mainly ELISA and Western Blotting), and, more recently, PCR-based methods for detecting prey DNA, particularly for the study of complex generalist predator-prey food-webs. The sensitivity of such methods, as well as specificity (capacity to avoid false positives) are high, however they are limited to detect particular target species. In particular, PCR methods need to differentiate the prey from the host DNA by employing species-specific primers unique to a single target species, which constitutes a great limitation to the approach. Recently developed next-generation sequencing (NGS) technologies provide a new possibility to detect prey species in DNA extracts without the need for PCR. Sequencing of total DNA of any specimen will also reveal a small portion of prey DNA, which is exploited here. However, rather than using any part of the genome, we specifically search for mitocondrial genes (mtDNA) which are naturally enriched over the nuclear genome due to the high number of mitochondria in each cell. Specifically, approximately 1% of all sequencing reads are derived from mitochondrial genomes, and we test here if this is sufficient to detect DNA that is derived from prey remaining in predator guts. In this talk we will present a case study to detect mtDNA in total genomic DNA extracts of predators, as the most promising molecular tool for analysis of prey diversity consumed and to construct food webs of predators in agroecosystems.  aDNA barcoding  ametagenomics  aMtDNA1 aVOGLER, A. P.