01743naa a2200217 a 450000100080000000500110000800800410001910000220006024501450008226000090022752009780023665000270121465000260124165300180126770000190128570000220130470000190132670000200134570000180136577301420138319830152017-07-19       2013    bl uuuu     u00u1 u    #d1 aBARÓ-ROBAINA, Y.  aCharacterization of Bacillus thuringiensis strains under the umbrella of Brazil-Cuba cooperatíon on bioinsecticides.h[electronic resource]  c2013  aAbstract: The crystal morphology and Cry protein composition of twelve Cuban Bacillus thuringiensis (Bt) strains were analyzed. Their in vitro biological activities were evaluated against Spodoptera frugiperda, Anticarsia gemmatalis and eggs of the nematode Meloidogyne incognita. The scanning electron micrographies as well as the morphology, size and number of the inclusions showed variations amongst the different strains, as expected. The Cry protein SDS-P AGE profile showed proteins of 130 kDa and 70 kDa. The bioassays showed two Bt strains highly active against S. [rugiperda and A. gemmatalis, and six strains interrupted the development of nematode egg mass. There were different behaviors regarding nematode infectivity but all of them resulted in nematostatic and disorientation effects under the experimental conditions. Based on the results obtained the two most virulent strains wiII be further studied for mass production and formulation as a biopesticide.  aBacillus thuringiensis  aInseticida bacteriano  aBioinseticida1 aMARQUES, M. E.1 aCAPALBO, D. M. F.1 aMELO, I. S. de1 aLARREA-VEGA, O.1 aMORAES, I. O.  tIn: MENDEZ-VILAS, A. (Ed.). Microbes in applied research: current advances and challenges. Singapore: World Scientific, 2012. p. 505-508.