01463naa a2200217 a 450000100080000000500110000800800410001910000200006024501080008026000090018850001570019752006750035465000140102965000180104365300270106165300090108865300170109770000160111470000220113077300930115219818492023-03-30       2013    bl uuuu     u00u1 u    #d1 aNOGUEIRA, G. F.  aSurvival of sugarcane shoot tips after cryopreservation by droplet-vitrificationh[electronic resource]  c2013  aNotas científicas. Título em português: Sobrevivência de ápices caulinares de cana-de-açúcar após criopreservação por ?droplet-vitrification?.  aThe objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet?vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L?1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L?1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.  aSaccharum  aRegeneração  aConservação in vitro  aPVS2  aRegeneration1 aPASQUAL, M.1 aPEREIRA, J. E. S.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 48, n. 11, p. 1524-1527, nov. 2013.