02555naa a2200253 a 450000100080000000500110000800800410001902400550006010000180011524501720013326000090030552017240031465000140203865000170205265300200206965300240208965300170211370000170213070000220214770000200216970000260218970000180221577300680223319721482024-02-05       2013    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.jnutbio.2013.08.0062DOI1 aPENEDO, L. A.  aIntake of butter naturally enriched with cis9, trans11 conjugated linoleic acid reduces systemic inflammatory mediators in healthy young adults.h[electronic resource]  c2013  aA conjugated linoleic acid (CLA) depletion-repletion study was carried out to investigate the effects of dietary c9,t11 CLA on C-reactive protein, transcription factor NFκB, metalloproteinases 2 and 9, inflammatory mediators (adiponectin, TNFα, IL-2, IL-4, IL-8, IL-10), body composition, and erythrocyte membrane composition in healthy normal-weight human adults. CLA depletion was achieved through an 8-week period of restricted dairy fat intake (depletion phase; CLA intake was 5.2±5.8 mg/day), followed by an 8-week period in which individuals consumed 20 g/day of butter naturally enriched with c9,t11 CLA (repletion phase; CLA intake of 1020±167 mg/day). The participants were 29 healthy adult volunteers (19 women and 10 men, aged 22 to 36 years), with body mass index between 18.0 and 29.9 kg m(-2). Blood samples were collected at baseline and at the end of both depletion and repletion phases. The content of CLA in erythrocytes decreased during CLA-depletion and increased during CLA-repletion. Intake of CLA-enriched butter increased the serum levels of anti-inflammatory IL-10 but reduced transcription factor NFκB in blood and serum levels of TNFα, IL-2, IL-8 and inactive metalloproteinase-9. Moreover, reduced activity of metalloproteinases 2 and 9 in serum was observed during the CLA-repletion period. In contrast, intake of CLA-enriched butter had no effects on body composition (DXA analysis) as well as on serum levels of adiponectin, C-reactive protein, and IL-4. Taken together, our results indicate that the intake of a c9,t11 CLA-enriched butter by normal-weight subjects induces beneficial changes in immune modulators associated with sub-clinical inflammation in overweight individuals.  acytokines  ainflammation  aBioactive lipid  aDepletion-repletion  aRumenic acid1 aNUNES, J. C.1 aGAMA, M. A. S. da1 aLEITE, P. E. C.1 aQUIRICO-SANTOS, T. F.1 aTORRES, A. G.  tJournal of Nutritional Biochemistrygv. 24, p. 2144-2151, 2013.