01694naa a2200229 a 450000100080000000500110000800800410001910000170006024500970007726000090017452010540018365000100123765300230124765300240127065300090129470000220130370000180132570000160134370000230135970000250138277300570140719716622022-10-31       2013    bl uuuu     u00u1 u    #d1 aSETTE, C. B.  aFormation and identification of PAHs metabolites in marine organisms.h[electronic resource]  c2013  aIn this study, the development of the technique APCIþ LC/MS/MS allowed the detection of phenanthrene, pyrene and metabolites of alkyl homologs in fish bile (in situ) and in urine of crabs. Laboratory experiments were carried out exposing crabs from an unpolluted mangrove (Barra de Guaratiba) to phenanthrene, and to the alkylated homologs 1-methyl phenanthrene and 2,6,9-trimethyl phenanthrene. Urine samples were collected at 0, 24, 48, 72, and 96 h. Fishes were captured from strategic sites from Guanabara Bay. Hydroxylated metabolites of phenanthrene, epoxides, orthoquinone and glucoside conjugates were identified in both samples. The method APCIþ LC/MS/MS showed to be effective in a preliminary assessment of phenanthrene metabolite formation, although the low concentrations of 1-methyl phenanthene and 2,6,9-trimethyl phenanthrene did not allow a systematic evaluation of data. The method, however, proved to be excellent tool for studies of PAHs metabolites due to the high selectivity, sensitivity and separation attained.  aPeixe  aBaía de Guanabara  aOrganismos marinhos  aPAHs1 aPEDRETE, T. de A.1 aFELIZZOLA, J.1 aNUDI, A. H.1 aSCOFIELD, A. de L.1 aWAGENER, A. de L. R.  tMarine Environmental Researchgv. 91, p. 2-13, 2013.