01643naa a2200265 a 450000100080000000500110000800800410001910000200006024500930008026000090017350001170018252007580029965000270105765000120108465000220109665000180111865000240113670000310116070000170119170000200120870000180122870000200124670000210126677300900128719705432017-06-19       2013    bl uuuu     u00u1 u    #d1 aATTÍLIO, L. B.  aGenetic transformation of sweet oranges with the D4E1 gene driven by tha AtPP2 promoter.  c2013  aTítulo em português: Transformação genética de laranjas doces com o gene D4E1 dirigido pelo promotor AtPP2.  aThe objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co-cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real-time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.  aAntimicrobial peptides  aOranges  aTransgenic plants  aLaranja pêra  aPlanta transgênica1 aMOURÃO FILHO, F. de A. A.1 aHARAKAVA, R.1 aSILVA, T. L. da1 aMIYATA, L. Y.1 aSTIPP, L. C. L.1 aMENDES, B. M. J.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 48, n. 7, p. 741-747, jul. 2013.