02106naa a2200361 a 450000100080000000500110000800800410001910000210006024500900008126000090017152011160018065000220129665000280131865000100134665000100135665000110136665300260137765300270140365300150143065300260144565300150147165300130148665300250149970000160152470000190154070000170155970000200157670000160159670000190161270000140163170000200164577300790166519675422013-10-01       2013    bl uuuu     u00u1 u    #d1 aRODRIGUES, A. G.  aBiogenic antimicrobial silver nanoparticles produced by fungi.h[electronic resource]  c2013  aAspergillus tubingensis and Bionectria ochroleuca showed excellent extracellular ability to synthesize silver nanoparticles (Ag NP), spherical in shape and 35± 10 nm in size. Ag NP were characterized by transmission electron microscopy, X-ray diffraction analysis, and photon correlation spectroscopy for particle size and zeta potential. Proteins present in the fungal filtrate and in Ag NP dispersion were analyzed by electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Ag NP showed pronounced antifungal activity against Candida sp, frequently occurring in hospital infections, with minimal inhibitory concentration in the range of 0.11-1.75 ?g/mL. Regarding antibacterial activity, nanoparticles produced by A. tubingensis were more effective compared to the other fungus, inhibiting 98.0 % of Pseudomonas. aeruginosa growth at 0.28 ?g/mL. A. tubingensis synthesized Ag NP with surprisingly high and positive surface potential, differing greatly from all known fungi. These data open the possibility of obtaining biogenic Ag NP with positive surface potential and new applications.  aAntifungal agents  aAspergillus tubingensis  aFungi  aFungo  aMangue  aAção antimicrobiana  aAntimicrobial activity  aBionectria  aBionectria ochroleuca  aCandida sp  aMangrove  aSilver nanoparticles1 aPING, L. Y.1 aMARCATO, P. D.1 aALVES, O. L.1 aSILVA, M. C. P.1 aRUIZ, R. C.1 aMELO, I. S. de1 aTASIC, L.1 aSOUZA, A. O. de  tApplied Microbiology and Biotechnology, New Yorkgv. 97, p. 775-782, 2013.