01890naa a2200325 a 450000100080000000500110000800800410001902200140006010000240007424501040009826000090020250001660021152008460037765000120122365000110123565000220124665000230126865000190129165300470131065300190135770000170137670000140139370000130140770000200142070000200144070000140146070000140147470000190148877300570150719620462013-07-15 2013 bl uuuu u00u1 u #d a0567-75721 aRODRIGUEZ, M. A. D. aDetecting Fusarium oxysporum f. sp. cubense tropical race 4 in soil and symptomless banana tissues. c2013 aTrabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. Eds.: I. Van den Bergh et al. aTropical race 4 (TR4) of Fusarium oxysporum f. sp. cubense (Foc) is a quarantine pathogen in many banana-producing regions of the world. Preventing further dissemination and precluding incursions into areas where it has not been observed is critical for maintaining local and commercial banana production. Trading of symptomless but infected banana planting material, movement of machinery with adhering infested soil, and the use of traditional banana-based packing material facilitate the spread of Foc. A PCR-based detection tool for TR4 was used to analyze field samples from symptomatic and symptomless banana plants and soils collected in TR4-affected areas. Foc TR4 was detected in all infected samples by conventional or nested PCR analyses. The results will be used to develop and implement TR4 quarantine and management strategies. aBananas aBanana aCultura de tecido aHistologia vegetal aMal do Panamá aFusarium oxysporum f. sp. cubense tropical aPlant diseases1 aWAALWIJK, C.1 aMUTUA, P.1 aDALY, A.1 aCHANG, P. F. L.1 aCORCOLON, B. M.1 aPAIVA, L.1 aSOUZA, M.1 aKEMA, G. H. J. tActa Horticulturaegn. 986, p. 127-130, April, 2013.