02064naa a2200277 a 450000100080000000500110000800800410001910000220006024501080008226000090019050001520019952010450035165000220139665000160141865300240143465300260145865300220148465300270150665300410153365300270157465300320160170000210163370000210165470000210167577300900169619610562017-06-13       2013    bl uuuu     u00u1 u    #d1 aRIBEIRO, C. A. G.  aMolecular characterization of soybean cultivars by microsatellite markers with universal tail sequence.  c2013  aTítulo em português: Caracterização molecular de cultivares de soja por meio de marcadores  microssatélites com sequência de cauda universal.  aThe objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2?7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2?5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the etermination of genetic purity.  aGenetic variation  aGlycine Max  aCultivar protection  aDiversidade genética  aGenotyping method  aMétodo de genotipagem  aProbabilidade de identidade ao acaso  aProteção de cultivar  aRandom identity probability1 aTANURE, J. P. M.1 aMACIEL, T. E. F.1 aBARROS, E. G. de  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 48, n. 3, p. 270-279, mar. 2013.