01699naa a2200253 a 450000100080000000500110000800800410001902200140006010000210007424501220009526000090021752009580022665000200118465000130120465000140121765000230123165300180125470000230127270000150129570000200131070000260133070000260135677300630138219607792023-05-19       2013    bl uuuu     u00u1 u    #d  a0103-90161 aSILVA, A. dos S.  aMolecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers.h[electronic resource]  c2013  aFusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed.  aGenetic markers  aFusarium  aMaracujá  aMarcador Molecular  aPassion fruit1 aOLIVEIRA, E. J. de1 aHADDAD, F.1 aJESUS, O. N. de1 aOLIVEIRA, S. A. S. de1 aCOSTA, M. A. P. de C.  tScientia Agricolagv.70, n.2, p.108-115, March/April 2013.