01812naa a2200229 a 450000100080000000500110000800800410001902400300006010000180009024501180010826000090022652011320023565000170136765000120138465300090139670000210140570000230142670000190144970000170146870000210148577300760150619590032023-05-02       2013    bl uuuu     u00u1 u    #d7 a10.3390/ijms140243262DOI1 aCASTELANI, L.  aMolecular typing of mastitis-causing Staphylococcus aureus isolated from heifers and cows.h[electronic resource]  c2013  aStaphylococcus aureus is among the main etiologic agents of bovine mastitis. A total of 83 isolates of S. aureus from mammary glands of primiparous heifers were collected in the prepartum, calving and during lactation. For lactating cows, a total of 27 isolates of S. aureus from mammary glands were collected during lactation. The samples were taken in two dairy farms located in Sao Paulo State, Brazil. The highest frequency of S. aureus isolation in heifers was at the end of lactation. Strains were typified through Pulsed-field gel electrophoresis (PFGE) and grouped according to patterns of restriction enzyme SmaI. PFGE generated seven clonal profiles that were grouped into three different lineages, with the LA lineage being predominant and identified in heifers, as well as in the cows from the two regions studied. It was concluded that the cows showed a significant source of dispersion of S. aureus. At the first lactation the heifers were infected by the same clonal profiles of S. aureus which were isolated from multiparous lactating cows. The heifers were infected during milking over the months of lactation.  aepidemiology  aheifers  aPFGE1 aSANTOS, A. F. S.1 aMIRANDA, M. dos S.1 aZAFALON, L. F.1 aPOZZI, C. R.1 aARCARO, J. R. P.  tInternational Journal of Molecular Sciencesgv. 14, p. 4326-4333, 2013.