02542naa a2200349 a 450000100080000000500110000800800410001910000200006024501770008026000090025752015060026665000180177265000100179065000120180065000160181265000210182865000140184965000120186365000240187565300230189965300270192265300260194965300080197570000220198370000200200570000210202570000170204670000220206370000220208570000200210777300650212719572522024-01-17       2011    bl uuuu     u00u1 u    #d1 aFROTA, I. M. A.  aEffects of BMP-7 and FSH on the development of goat preantral follicles and levels of mRNA for FSH-R, BMP-7 and BMP receptors after in-vitro culture.h[electronic resource]  c2011  aAbstract: This study aimed to evaluate the effects of FSH and BMP-7 on growth and on expression of FSH-R, BMP-7 and BMP receptors in cultured secondary follicles. Goat secondary follicles (~200 µm) were isolated and cultured in vitro, with 5% CO2 in air at 39°C, for 6 days in the presence of BMP-7 (50 ng/ml) supplemented or not with FSH (50 ng/ml). Follicular diameter and the formation of the antrum were evaluated before and after culture. For each treatment, at the end of culture period, groups of 6 follicles were collected and, after extraction of total RNA and cDNA synthesis, the levels of mRNA for FSH-R, BMP-7 and BMP receptors in cultured secondary follicles were quantified by real time PCR. The results showed that addition of BMP-7 or FSH to culture medium stimulated growth of secondary follicles, while addition of both BMP-7 and FSH was needed to significantly increase the percentage of follicles forming an antrum and the follicular levels of mRNA for both BMP-7 and FSH-R. For BMP receptors, FSH reduced the levels of mRNA for BMPR-IA and BMP-RII in comparison with those follicles cultured in MEM alone and supplemented with BMP-7, respectively. In conclusion, like FSH, BMP-7 affects in vitro growth of cultured secondary follicles, but it stimulates antrum formation and expression of the mRNA?s for BMP-7 and FSH-R only in presence of both BMP-7 and FSH. However, the levels of mRNA for BMP-RIA and BMP-RII are reduced in follicles cultured in medium supplemented with FSH.  aBiotecnologia  aCabra  aCaprino  aFertilidade  aGenética animal  aHormônio  aOvário  aReprodução animal  aFolículo ovariano  aFolículos pre-antrais  aFolítulo pré-antral  aFSH1 aLEITÃO, C. C. F.1 aCOSTA, J. J. N.1 aHURK, R. van den1 aBRITO, I. R.1 aSARAIVA, M. V. A.1 aFIGUEIREDO, J. R.1 aSILVA, J. R. V.  tAnimal Reproductiongv. 8, n. 1/2, p. 25-31, Jan./Jun. 2011.