01924naa a2200253 a 450000100080000000500110000800800410001910000180006024501330007826000090021152011840022065000120140465000190141665000110143565000280144670000150147470000200148970000150150970000170152470000200154170000240156170000240158577300610160919539552016-07-08       2008    bl uuuu     u00u1 u    #d1 aLILENBAUM, W.  aDetection of Leptospira spp. in semen and vaginal fluids of goats and sheep by polymerase chain reaction.h[electronic resource]  c2008  aAbstract: Thirteen goat herds and seven sheep flocks in the state of Rio de Janeiro, Brazil were screened for leptospirosis. From the three herds and three flocks with greatest seroreactivity, 19 goats (16 females and three bucks) and 40 sheep (26 ewes and 14 rams) that were seropositive (specific anti-Leptospira titres '&gt OU ='400, based on a microscopic agglutination test), were selected for more detailed studies. From those animals, samples of vaginal fluids or semen were collected for bacteriological and molecular assays. For both species of animals, the most prevalent reactions were to serovars Hardjo, Shermani, and Grippotyphosa. Although leptospires were detected by darkfield microscopy in three vaginal fluid samples (from two goats and one ewe), pure isolates were not obtained by bacteriological culture of vaginal fluids or semen. However, seven vaginal fluid samples (from four goats and three ewes) and six semen samples (all from rams) were positive on polymerase chain reaction (PCR). Based on these findings, in addition to analogous findings in cattle, we inferred that there is potential for venereal transmission of leptospirosis in small ruminants.  aCaprino  aDoença animal  aOvelha  aTransmissão de doença1 aVARGES, R.1 aBRANDÃO, F. Z.1 aCORTEZ, A.1 aSOUZA, S. O.1 aBRANDÃO, P. E.1 aRICHTZENHAIN, L. J.1 aVASCONCELLOS, S. A.  tTheriogenology, New Yorkgv. 69, n. 7, p. 837-842, 2008.