02303naa a2200277 a 450000100080000000500110000800800410001910000240006024501100008426000090019430000200020350000130022352013130023665000140154965000100156365300200157365300250159370000200161870000160163870000230165470000230167770000180170070000200171870000230173877302640176119534042013-03-19       2011    bl uuuu     u00u1 u    #d1 aBARBOSA-FERREIRA, M  aAntinutritional factors in plants. Steroidal saponin (Protodioscin) in Brachiraria spp. Preliminary data.  c2011  a11 p.c1 CD-ROM  aIII SIMF  aOutbreaks of hepatogenous photosensitization have been reported in ruminants grazing Brachiraria spp. Saponins are secondary metabolites present in that grass, and these substances are related as toxic causing the disease. The objective of this study was to identify and quantify the variations of the saponin protodioscin content in green leaves of different Brachiraria species/ecotypes. Samples of leaves were collected from 10 ecotypes of Brachiraria spp., from August 2009 to August 2010. Following the procedure of extracting saponins from the dried and crushed leaves, the analysis was performed using HPLC-ELSD for identification and quantification of protodioscin. It was observed that the species B. decumbens has the highest concentration of protodioscin (2.33% ± 0.54), B. brizantha var. Arapoty has the highest concentration (1.24% ± 0.44) between the B. brizantha ecotypes. Finally B. humidicola cultivars have the lowest concentrations ranging from 0.11 to 0.9%. These data confirm the observations of other researchers who cite B. decumbens as responsible for most cases of hepatogenous photosensitization. It can be concluded that the varieties of B. decumbens are those with the highest concentrations of protodioscin, followed by varieties of B. brizantha and varieties of B. humidicola.  apoisoning  asheep  aHepatic disease  aSecondary metabolite1 aVALLE, C. B. do1 aBRUM, K. B.1 aOLIVEIRA, N. M. R.1 aFERREIRA, V. B. N.1 aGARCEZ, V. S.1 aRIET-CORREA, F.1 aLEMOS, R. A. A. de  tIn: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forages for climate change adaptation and mitigation - eco-efficient animal production: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. 1 CD-ROM. III SIMF. p 322-332.