01577naa a2200205 a 450000100080000000500110000800800410001910000200006024501930008026000090027352008950028265000230117765000270120065000260122765300170125370000160127070000220128670000210130877300420132919471722025-07-04       1999    bl uuuu     u00u1 u    #d1 aSOUZA, M. T. de  aUltrastructural and molecular characterization of the Parasporal Crystal Proteins of Bacillus thuringiensis subsp. kurstaki S93 active against Spodoptera frugiperda.h[electronic resource]  c1999  aA Brazilian strain of Bacillus thuringiensis subsp. kurstaki (S93), which was previously shown to be more toxic to Spodoptera frugiperda than the commercially available strain HDI DIPEL, was characterized. The S93 isolate was shown to produce bypiramidal and cuboidal shape crystals. SDS-PAGE of sporulating cultures showed the presence of mainly two polypeptides of ca. 135- and 65-kDa, respectively. The presence of these polypeptides was in agreement with the type of crystals found. crylA- and cryIIA-type genes were also detected in the plasmidial DNA from this strains by using PCR. Furthermore, plasmidial profile of this strain has a very similar content to the HD1 DIPEL strain. These results hint that the Brazilian strain is very similar to the HD1 in several aspects. However, the reasons for the increased activity of the Brazilian isolate towards S. frugiperda remain unknown.  abiological control  aBacillus Thuringiensis  aSpodoptera Frugiperda  aCry proteins1 aLIMA. M. I.1 aWERNECK, J. O. S.1 aDIAS, J. M. C. S  tBiocellgv. 23, n. 1, p. 43-49, 1999.