02163naa a2200241 a 450000100080000000500110000800800410001902400170006010000190007724501130009626000090020952014160021865000090163470000170164370000220166070000240168270000210170670000180172770000200174570000250176570000160179077301150180619452132016-03-04       2012    bl uuuu     u00u1 u    #d7 a10.10992DOI1 aRIBEIRO, R. A.  aReclassification of Rhizobium tropical type A strains as Rhizobium leucaenae sp. nov.h[electronic resource]  c2012  aRhizobium tropici is a well-studied legume symbiont characterized by high genetic stability of the symbiotic plasmid and tolerance to tropical environmental stresses such as high temperature and low soil pH. However, high phenetic and genetic variabilities among R. tropici strains have been largely reported, with two subgroups, designated type A and B, already defined within the species. A polyphasic study comprising multilocus sequence analysis, phenotypic and genotypic characterizations, including DNA?DNA hybridization, strongly supported the reclassification of R. tropici type A strains as a novel species. Type A strains formed a well-differentiated clade that grouped with R. tropici, Rhizobium multihospitium, Rhizobium miluonense, Rhizobium lusitanum and Rhizobium rhizogenes in the phylogenies of the 16S rRNA, recA, gltA, rpoA, glnII and rpoB genes. Several phenotypic traits differentiated type A strains from all related taxa. The novel species, for which the name Rhizobium leucaenae sp. nov. is proposed, is a broad host range rhizobium being able to establish effective root-nodule symbioses with Leucaena leucocephala, Leucaena esculenta, common beans (Phaseolus vulgaris) and Gliricidia sepium. Strain CFN 299 T (5USDA 9039 T 5LMG 9517 T 5CECT 4844 T 5JCM 21088 T 5IAM 14230 5SEMIA 4083 T 5CENA 183 T 5UMR1026 T 5CNPSo 141 T ) is designated the type strain of Rhizobium leucaenae sp. nov.  aSoja1 aROGEL, M. A.1 aLÓPEZ-LÓPEZ, A.1 aORMEÑO-ORRILLO, E.1 aBARCELLOS, F. G.1 aMARTÍNEZ, J.1 aTHOMPSON, F. L.1 aMARTÍNEZ-ROMERO, E.1 aHUNGRIA, M.  tInternational Journal of Systematic and Evolutionary Microbiology, Readinggv. 62 , Pt. 5, p. 1179-1184, 2012.