01754naa a2200289 a 450000100080000000500110000800800410001910000180006024501480007826000090022652009370023565000110117265000220118365000250120565000130123065000190124365000080126265000100127065300220128065300160130270000160131870000190133470000200135370000190137370000200139277300520141219444172013-01-08       2012    bl uuuu     u00u1 u    #d1 aBARROS, E. P.  aDevelopment and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines.  c2012  aAn automated head space solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, no risoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time,and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5ml of wine with 2gNaCl at 45 1C during 5 min, and subsequent extraction of min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile composition of different white wines.  aGrapes  aAnálise química  aCromatografia Gasosa  aEnologia  aEspectrometria  aUva  aVinho  aComposto volátil  aMetodologia1 aMOREIRA, N.1 aPEREIRA, G. E.1 aLEITE, S. G. L.1 aREZENDE, C. M.1 aPINHO, P. G. de  tTalanta, Oxfordgv. 101, p. 177-186, nov. 2012.