02495naa a2200265 a 450000100080000000500110000800800410001910000180006024501400007826000090021852016520022765300290187965300380190865300370194665300200198365300190200370000190202270000240204170000190206570000220208470000150210670000250212170000280214677300550217419422372023-03-24       2012    bl uuuu     u00u1 u    #d1 aLAPORT, M. S.  aAntimicrobial activity of marine sponges against coagulase-negative staphylococci isolated from bovine mastitis.h[electronic resource]  c2012  aBovine mastitis remains worldwide a major challenge for the dairy industry despite the widespread implementation of control strategies. The increasing number of coagulasenegative staphylococci (CNS) causing mastitis and of bacteria resistant to conventional antibiotics has become a serious problem in recent years. Marine sponges are a rich source of bioactive compounds, and many species can be useful for the development of new antimicrobial drugs. In the present study, 49 CNS strains were isolated from bovine mastitis cases from 21 different dairy herds kept at farms in Southeast Brazil. Strains were analyzed for antimicrobial susceptibility and mecA gene detection. Fifty-nine percent of the CNS strains were resistant to at least one of the drugs tested and 12.2% were classified as multiresistant. Three strains carried the mecA gene, confering resistance to the betalactamic antibiotics. In addition, the CNS strains were submitted to in vitro screening for antimicrobial activities of extracts from marine sponges. Extracts from the sponge species Cinachyrella sp., Haliclona sp. and Petromica citrina showed antibacterial activity against 61% of the CNS strains, including strains resistant to conventional antibiotics. Extracts from P. citrina showed the largest spectrum of inhibitory activity. The aqueous extract inhibited 51% of the CNS strains and presented a bactericidal effect over susceptible and multiresistant-bacteria at a minimal inhibitory concentration of 1.024 mg/ml. This study shows the potential of marine sponges as new sources of antibiotics and disinfectants for the control of CNS involved in bovine mastitis.  aAtividade antibacteriana  aBacteria resistente a antibiotico  aCoagulase-negative staphylococci  aEsponja marinha  aMastite bovina1 aMARINHO, P. R.1 aSANTOS, O. C. DA S.1 aALMEIDA, P. DE1 aROMANOS, M. T. V.1 aMURICY, G.1 aBRITO, M. A. V. P. e1 aGIAMBIAGI-DE-MARVAL, M.  tVeterinary Microbiologygv. 155, p. 362-368, 2012.