03045naa a2200265 a 450000100080000000500110000800800410001902400550006010000180011524501700013326000090030352021800031265000200249265000120251265300250252465300250254965300170257470000180259170000200260970000170262970000190264670000240266570000230268977300670271219402492023-03-10       2013    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.106/j.vetpar.2012.09.30.2DOI1 aKATITI, L. M.  aAnthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity.h[electronic resource]  c2013  aAlthough tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (CT), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (HT). We evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either mostly CT, mostly HT, or both CT and HT. Extracts were prepared with 70% acetone, lyophilized, redissolved to doses ranging from 1.0 mg/mL to 25 mg/mL, and tested against adult Caenorhabditis elegans as a test model. The extract concentrations that killed 50% (LC50) or 90% (LC90) of the nematodes in 24 h were determined and compared to the veterinary anthelmintic levamisole (8 mg/mL). Extracts were quantified for CT by the acid butanol assay, for HT (based on gallic acid and ellagic acid) by high-performance liquid chromatography (HPLC) and total phenolics, and for their antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay. Extracts with mostly CT were Lespedeza cuneata, Salix X sepulcralis, and Robinia pseudoacacia. Extracts rich in HT were Acer rubrum, Rosa multiflora, and Quercus alba, while Rhus typhina had both HT and CT. The extracts with the lowest LC50 and LC90 concentrations, respectively, in the C. elegans assay were Q. alba (0.75 and 1.06 mg/mL), R. typhina collected in 2007 (0.65 and 2.74 mg/mL), A. rubrum (1.03 and 5.54 mg/mL), and R. multiflora (2.14 and 8.70 mg/mL). At the doses of 20 and 25 mg/mL, HT-rich, or both CT- and HT-rich, extracts were significantly more lethal to adult C. elegans than extracts containing only CT. All extracts were high in antioxidant capacity, with ORAC values ranging from 1800 moles to 4651 moles of trolox equivalents/g, but ORAC did not correlate with anthelmintic activity. The total phenolics test had a positive and highly significant (r = 0.826, p ≤ 0.01) correlation with total hydrolyzable tannins. Plants used in this research are naturalized to the Appalachian edaphoclimatic  aSmall ruminants  aTannins  aAntioxidant capacity  aHydrolyzable tannins  aTree tannins1 aJORGES, F. S.1 aGONZALEZ, J. M.1 aZAJAC, A. M.1 aLINDSAY, D. S.1 aCHAGAS, A. C. de S.1 aAMARANTE, A. F. T.  tVeterinary Parasitlogygv. 192, n. 1-3, p. 218-227, feb. 2013.