01660naa a2200217 a 450000100080000000500110000800800410001910000300006024501100009026000090020052010120020965000160122165300140123765300330125170000190128470000220130370000160132570000210134170000170136277300630137919392042013-04-24       2012    bl uuuu     u00u1 u    #d1 aPIMENTA-MARTINS, M. G. R.  aDevelopment of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese.  c2012  aThis paper reports a novel electrochemical immunosensorforthe sensitive detection of staphylococcal enterotox in A (S EA ) based on self-assembly monolayer (SA M) and p rotein A i mm obilizat io n on gold electro de. Three diffe rent methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modi fied gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and speci ficity. The proposed amperometric immuno- sensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses.  afood safety  aBiosensor  aStaphylococcal enterotoxin A1 aFURTADO, R. F.1 aHENEINE, L. G. D.1 aDIAS, R. S.1 aBORGES, M. de F.1 aALVES, C. A.  tJournal of Microbiological Methodsgv.91, p.138-143, 2012.