01325naa a2200217 a 450000100080000000500110000800800410001902200140006002400350007410000170010924501010012626000090022752006940023665000150093065000110094570000190095670000160097570000240099170000190101577300730103419347412018-05-24       2012    bl uuuu     u00u1 u    #d  a0304-86087 a10.1007/s00705-011-1196-42DOI1 aHALLWASS, M.  aSequence determination and analysis of the NSs genes of two tospoviruses.h[electronic resource]  c2012  aThe tospoviruses groundnut ringspot virus (GRSV) and zucchini lethal chlorosis virus (ZLCV) cause severe losses in many crops, especially in solanaceous and cucurbit species. In this study, the non-structural NSs gene and the 50UTRs of these two biologically distinct tospoviruses were cloned and sequenced. The NSs sequence of GRSV and ZLCV were both 1,404 nucleotides long. Pairwise comparison showed that the NSs amino acid sequence of GRSV shared 69.6% identity with that of ZLCV and 75.9% identity with that of TSWV, while the NSs sequence of ZLCV and TSWV shared 67.9% identity. Phylogenetic analysis based on NSs sequences confirmed that these viruses cluster in the American clade.  aTospovirus  aVírus1 aLEASTRO, M. O.1 aLIMA, M. F.1 aINOUE-NAGATA, A. K.1 aRESENDE, R. O.  tArchives of Virology, New Yorkgv. 157, n. 3, p. 591-596, Mar. 2012.