01390naa a2200205 a 450000100080000000500110000800800410001902400320006010000170009224501750010926000090028452007040029365000180099770000250101570000180104070000220105870000260108070000160110677300620112219329032017-08-03       2012    bl uuuu     u00u1 u    #d7 a10.1002/pmic.2011004062DOI1 aGOMES, D. F.  aTwo-dimensional proteome reference map of Rhizobium tropici PRF 81 reveals several symbiotic determinants and strong resemblance with agrobacteria.h[electronic resource]  c2012  aRhizobium tropici strain PRF 81 is used in commercial inoculants for common-bean crops in Brazil because of its high efficiency in nitrogen fixation and, as in other strains belonging to this species, its tolerance of environmental stresses, representing a useful biological alternative to chemical nitrogen fertilizers. In this study, a proteomic reference map of PRF 81 was obtained by two-dimensional gel electrophoresis and MALDI-TOF/TOF-TOF mass spectrometry. In total, 115 spots representing 109 different proteins were successfully identified, contributing to a better understanding of the rhizobia-legume symbiosis and supporting, at proteomics level, a strong resemblance with agrobacteria.  aMicrobiologia1 aBATISTA, J. S. da S.1 aTORRES, A. R.1 aANDRADE, D. de S.1 aGALLI-TERASAWA, L. V.1 aHUNGRIA, M.  tProteomics, Weinheimgv. 12, n. 6, p. 859-863, mar. 2012.