01862naa a2200289 a 450000100080000000500110000800800410001910000210006024501740008126000090025552009230026465000220118765000260120965000220123565000240125765000220128165000180130365000220132165000130134365000280135670000230138470000280140770000210143570000190145670000200147577300770149519328152012-12-20       2012    bl uuuu     u00u1 u    #d1 aHASHIMOTO, J. C.  aA simple method for the determination of malachite green and leucomalachite green residues in fish by a modified QuEChERS extraction and LC/MS/MS.h[electronic resource]  c2012  aA simple method using HPLC/MS/MS was developed and validated to determine residues of malachite green (MG) and leucomalachite green (LMG) in fish fillet. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique was used to perform the sample preparation. The optimal extraction and cleanup conditions were established using an experimental design. The validation parameters obtained to determine both MG and LMG complied with the requirements established by regulatory agencies for the presence of such substances in fish, which establish that the method must attain, at least, a minimum required performance limit of 2 ng/g. The accuracy values ranged etween 95 and 107%, and the precision values were lower than 11.2%. The method was used in the analysis of tilapia samples (n = 20) commercialized in Campinas, SP, Brazil. None of the samples presented detectable levels of MG or LMG residues.  aChemical residues  aLiquid chromatography  aMass spectrometry  aTilapia (Cichlidae)  aAnálise química  aCromatografia  aResíduo químico  aTilápia  aToxicologia de alimento1 aPASCHOAL, J. A. R.1 aQUEIROZ, S. C. do N. de1 aFERRACINI, V. L.1 aASSALIN, M. R.1 aREYES, F. G. R.  tJournal of AOAC International, Arlingtongv. 95, n. 3, p. 913-922, 2012.