02283naa a2200289 a 450000100080000000500110000800800410001902200140006002400530007410000170012724501290014426000090027352013580028265000250164065000170166565300150168270000230169770000200172070000180174070000200175870000240177870000240180270000210182670000230184770000210187077301020189119285022021-10-07       2011    bl uuuu     u00u1 u    #d  a0147-95717 ahttps://doi.org/10.1016/j.cimid.2011.08.0032DOI1 aMONTE, L. G.  aMonoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions.h[electronic resource]  c2011  aLeptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis.  aAnticorpo Monoclonal  aLeptospirose  aMonoclonal1 aCONCEIÇÃO, F. R.1 aCOUTINHO, M. L.1 aSEIXAS, F. K.1 aSILVA, E. V. da1 aVASCONCELLOS, F. A.1 aCASTRO, L. A. S. de1 aHARTLEBEN, C. P.1 aDELLAGOSTIN, O. A.1 aALEIXO, J. A. G.  tComparative Immunology, Microbiology and Infectious Diseasesgv. 34, n. 5, p. 441-446, set. 2011.