02964naa a2200481 a 450000100080000000500110000800800410001902200460006002400350010610000180014124501220015926000090028152014560029065000210174665000100176765000130177765000210179065000330181165000340184465000210187865000220189965000100192165000140193165000140194565000210195965000330198065000240201365000250203765300210206265300460208365300150212965300400214465300220218465300230220665300350222965300250226470000170228970000190230670000260232570000220235170000200237377300890239319164872021-07-06 2012 bl uuuu u00u1 u #d a0167-6857 (impresso) / 1573-5044 (online)7 a10.1007/s11240-011-0009-52DOI1 aFLORES, P. S. aIn vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt.h[electronic resource] c2012 aFusarium vascular wilt (caused by Fusarium oxysporum f. sp. passiflorae) is a limiting factor in the cultivation of yellow passion fruit (Passiflora edulis). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either Fusarium oxysporum f. sp. passiflorae (FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by F. oxysporum. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the Fusarium vascular wilt. aAgronomic traits aFungi aGenotype aIn vitro culture aPlant diseases and disorders aCaracterísticas Agronômicas aCultura in vitro aDoença de planta aFungo aFusariose aGenótipo aMaracujá Peroba aPassiflora edulis flavicarpa aSeleção genótipa aVariedade resistente aCultivo in vitro aEnfermedades y desórdenes de las plantas aFusariosis aFusarium oxysporum f sp passiflorae aMaracujá amarelo aMaracuyá amarillo aPassiflora edulis f flavicarpa aYellow passion fruit1 aOTONI, W. C.1 aDHINGRA, O. D.1 aDINIZ, S. P. S. de S.1 aSANTOS, T. M. dos1 aBRUCKNER, C. H. tPlant Cell, Tissue and Organ Culture, Heidelberggv. 108, n. 1, p. 37-45, Jan. 2012.