03075naa a2200553 a 450000100080000000500110000800800410001910000200006024501240008026000090020452014800021365000100169365000240170365000200172765000250174765000220177265000160179465000250181065000180183565000120185365000210186565000110188665000230189765000230192065000250194365000260196865000230199465000270201765000250204465000220206965300270209165300230211865300260214165300130216765300120218065300260219265300260221865300200224465300320226465300220229665300250231870000140234370000190235770000180237670000180239470000130241270000200242577300760244519025852021-07-06       2011    bl uuuu     u00u1 u    #d1 aSOUSA, A. C. B.  aGenetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers.  c2011  aThe pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47, respectively, showing a deficit in heterozygosity. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign genotypes into clusters. A dendrogram was constructed based on the modified Roger's genetic distances using a neighbor-joining method (NJ). A total of four clusters were assembled by STRUCTURE and a strong tendency of correspondence between the Bayesian clusters in the NJ tree was observed. The genetic distance ranged from 0.09 to 0.62 (average 0.37), showing a low genetic diversity in the pigeonpea genotypes. Transferability of pigeonpea-specific microsatellites revealed a cross-amplification and the presence of polymorphic alleles in P. vulgaris and V. unguiculata.  aBeans  aComputer simulation  aGenetic markers  aGenetic polymorphism  aGenetic variation  aPigeon peas  aStatistical analysis  aCajanus cajan  aFeijão  aFeijão de corda  aGuandu  aMarcador genético  aMarcador molecular  aMétodo estatístico  aModelo de simulação  aPhaseolus vulgaris  aPolimorfismo genético  aVariação genética  aVigna unguiculata  aAnálisis estadístico  aBayesian inference  aDiversidade genética  aFrijoles  aGuandul  aInferência bayesiana  aMarcadores genéticos  aMicrossatélite  aSimulación por computadora  aSofware Structure  aVariación genética1 aGODOY, R.1 aSFORÇA, D. A.1 aCAMPOS, T. de1 aZUCCHI, M. I.1 aJANK, L.1 aSOUZA, A. P. de  tScientia Agricola, Piracicabagv. 68, n. 4, p. 431-439, July/Aug. 2011.