02153naa a2200265 a 450000100080000000500110000800800410001902400570006010000240011724501330014126000090027452012660028365000140154965000160156365300170157965300130159665300360160970000210164570000230166670000260168970000210171570000180173670000250175477301080177918894632022-07-27 2011 bl uuuu u00u1 u #d7 ahttps://doi.org/10.1590/S1984-296120110001000072DOI1 aCHAGAS, A. C. de S. aIn vitro activity of Artemisia annua L (Asteraceae) extracts against Rhipicephalus (Boophilus) microplus.h[electronic resource] c2011 aThe activity of plant extracts on parasites may indicate groups of substances that are potentially useful for controlling Rhipicephalus (Boophilus) microplus. The aim of the present study was to investigate the in vitro action of Artemisia annua extracts on this tick. The concentrations of the sesquiterpene lactones artemisinin and deoxyartemisinin present in plant extracts were quantified via high-performance liquid chromatography. Four extracts produced from the concentrated crude extract (CCE) were evaluated on larvae using the impregnated paper method, with readings after 24 hours of incubation. The engorged females were immersed in the CCE and in its four derived extracts for five minutes, with incubation for subsequent analysis of biological parameters. The extracts were not effective on the larvae at the concentrations tested (3.1 to 50 mg.mL-1). The CCE showed greater efficacy on engorged females (EC50 of 130.6 mg.mL-1 and EC90 of 302.9 mg.mL-1) than did the derived extracts. These results tend to confirm that the action of artemisinin on engorged females of R.(B.) microplus is conditional to their blood intake. In this case, in vitro methods would be inadequate for effective evaluation of the action of A. annua on R. (B.) microplus. aCarrapato aFitoterapia aArtemisinina aControle aR (B) microplus Artemisia annua1 aGEORGETTI, C. S.1 aCARVALHO, C. O. de1 aOLIVEIRA, M. C. de S.1 aRODRIGUES, R. A.1 aFOGLIO, M. A.1 aMAGALHÃES, P. M. DE tRevista Brasileira de Parasitologia e Veterinária, Jaboticabalgv. 20, n. 1, p. 31-35, jan.-mar. 2011.