02402nam a2200205 a 450000100080000000500110000800800410001910000200006024501150008026001570019550000140035252017110036665000110207765300130208870000180210170000170211970000200213670000170215670000230217318869212011-04-29       2011    bl uuuu     u00u1 u    #d1 aJESUS, O. N. de  aIdentification of the genomic constitution and ploidy level of banana accessions using microsatellite markers.  aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.c2011  apdf 35265  aMost banana and plantain cultivars originated from the natural cross between the wild diploid species M. acuminata Colla (A genome) and M. balbisiana Colla (B genome) resulting in the following genomic combinations: AA, BB, AB, ABB, AAB, AAA, AAAB and AAAA. The definition of the ploidy and genomic constitution plays a key role in the classification of bananas and breeding strategies since most characteristics are associated to them. The ploidy level influences the phenology and yield parameters, while the genomic constitution affects chemical attributes of fruits with greater plasticity, sweetness and starch content; besides being related to biotic and abiotic resistance factors. The germplasm bank at Embrapa has many accessions from different origins, ploidy levels and genomic constitution, whereas the characterization has mainly been carried out using morphological descriptors. Among the molecular tools, microsatellite markers are highlighted due to their co-dominant nature, for being highly polymorphic and presenting high informative capacity. SSR markers have been used in bananas to establish ploidy levels and genomic constitution. Therefore, 16 SSR loci were used to characterize 224 banana accessions from the germplasm bank at Embrapa/CNPMF. Eight primers were selected for their combined use in the characterization of the A and B genomes whereas the MaCCEN06, MaOCEN12 and MaOCEN14 loci presented higher discrimination power. According to the cluster analysis data, 210 (94%) accessions were in agreement in regard to their passport information, 7 contradicted (3%), 3 (1.3%) had their genomic constitution defined and 4 (1.7%) were not determined in regard to the groups formed.  aBanana  aMusa spp1 aAMORIM, E. P.1 aSILVA, S. O.1 aFERREIRA, C. F.1 aSILVA, G. G.1 aFIGUEIRA, A. V. O.