01696nam a2200181 a 450000100080000000500110000800800410001910000240006024501040008426001980018852010190038665000110140565300170141670000170143370000210145070000220147170000210149318857612011-04-13       2007    bl uuuu     u00u1 u    #d1 aBUDZINSKI, I. G. F.  aCoffea expansin gene family and expansin expression during fruit maturation.h[electronic resource]  aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.c2007  aExpansins (EXP) are plant cell-wall loosing proteins involved in cell enlargement and developmental process such as organogenesis, seeds germination, cell wall dissolution and fruit ripening. Two families of EXP are known, α-expansin (EXPA), involved in the control of cell wall extension, and β-expansins (EXPB), the major allergens of grass pollen that also have cell wall-loosening activity. With the objective to study the role of EXP in coffee fruit maturation we selected EXP homologous sequences on the Brazilian Coffee Genome Project database. Full-length contigs were classified according the EXP family. Northern blots of pulp from fruits at the latest stages of maturation (22-32 weeks after flowering) showed increased transcription of one contig (CaEXP1). This higher transcription corresponds with a climacteric burst, during the expansion of pulp, with a decline in ?cherry? fruits. The transcription of CaEXP1 was also observed in roots, shoots, flower and flower buds, but not in leaves.  aCoffea  aOganogenesis1 aLEITE, T. F.1 aTAKAHASHI, E. K.1 aPEREIRA, L. F. P.1 aVIEIRA, L. G. E.