01299nam a2200229 a 450000100080000000500110000800800410001910000280006024501310008826001820021950000130040152004680041465300110088265300260089365300260091965300170094570000230096270000200098570000210100570000200102670000230104618841452025-07-11 2010 bl uuuu u00u1 u #d1 aCARDOZO FILHO, J. de L. aIdentification of a phylloseptin from Amazon Phyllomedusa palliata(AnurabHylidae) by Maldi TOT/TOF MS.h[electronic resource] aIn: CONGRESSO DA SOCIEDADE BRASILEIRA DE TOXINOLOGIA, 11., 2010, Araxá. Toxinas naturais: conhecimento atual e novos desafios. [S.l.]: Sociedade Brasileira de Toxinologiac2010 aPôster. aThis work aims to the characterization of peptides from nine specimens of Phyllomedusa palliata obtained in Cruzeiro do Sul/AC under the IBAMA authorization number 17983-2. The skin secretion was obtained by manual stimulation and washing using pure water followed by freezing and lyophilization of the wash solution. 2mg of crude secretion was resuspended in TFA 0.1% and chromatographed on a reverse phase C18 HPLC analytic column (Jupiter Proteo - Phenomenex). aAmazon aAntimicrobial peptide aPhyllomedusa palliata aPhylloseptin1 aMONTEIRO, R. do N.1 aSILVA, L. P. da1 aBLOCH JUNIOR, C.1 aSTÁBELI, R. G.1 aCALDERON, L. de A.