02078nam a2200193 a 450000100080000000500110000800800410001910000160006024501340007626000790021052014480028965000260173765300110176365300300177470000200180470000220182470000180184670000200186418800742011-03-04       2008    bl uuuu     u00u1 u    #d1 aSATO, J. H.  aComparative proteomic analysis of Coffea arabica somatic embryos in the different stages of embryogenesis.h[electronic resource]  aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.c2008  aCoffee is one of the most important cultivated agricultural crops and is of great importance for Brazilian economy. Studies focusing on somatic embryogenesis have been widely performed aiming at the comprehension of the mechanisms involved. Somatic embryogenesis is an important process that helps in vitro micropropagation, which is performed to obtain a high number of plants with high economic value, genetically uniform and free from diseases. The objective of this study was to analyze the protein profile of Coffea arabica cv. Catuaí Vermelho somatic embryos in the stages of torpedo and cotyledonary by two-dimensional electrophoresis (2-DE). Samples were collected and macerated in liquid nitrogen. Protein extraction was performed by using phenol and extraction buffer. The proteins obtained were quantified, analyzed by 2-DE and stained with silver nitrate. The 2D gels revealed proteins varying in size from 10 to 160 kDa and in pI from 3 to 10. The analysis of the protein profiles showed a higher amount of proteins in the cotyledonary phase, especially in the area of pH 3 to 5,5. A total of 45 differentially expressed proteins were observed when comparing both profiles, including 8 proteins observed only in the cotyledonary phase and 4 exclusive to the torpedo phase. Theses proteins will be further identified by mass spectrometry in order to better understand the expression changes during somatic embryogenesis in coffee.  aSomatic embryogenesis  aCoffee  aIn vitro micropropagation1 aTEIXEIRA, J. B.1 aEIRA, M. T. S. da1 aFRANCO, O. L.1 aREIS, A. M. dos