00993nam a2200181 a 450000100080000000500110000800800410001910000210006024500980008126001130017952004180029265000130071065000090072370000210073270000220075370000210077570000150079618740762025-06-09 2010 bl uuuu u00u1 u #d1 aKULCHESKI, F. R. aThe use of microRNAs as universal reference genes in quantitative PCR.h[electronic resource] aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... [Curitiba]: UFPR, 2010. p. 312.c2010 aReverse transcription quantitative real-time PCR (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. aSoybeans aSoja1 aMARCELINO, F. C.1 aNEPOMUCENO, A. L.1 aABDELNOOR, R. V.1 aMARGIS, R.