01808naa a2200253 a 450000100080000000500110000800800410001902400350006010000210009524501530011626000090026952010220027865000170130065000150131765000170133265000150134965000120136470000210137670000160139770000190141370000260143270000210145877300750147918725392025-05-19       2011    bl uuuu     u00u1 u    #d7 a10.1016/j.etp.2010.03.0052DOI1 aMARCARINI, J. C.  aInvestigation of cytotoxic, apoptosis-inducing, genotoxic and protective effects of the flavonoid rutin in HTC hepatic cells.h[electronic resource]  c2011  aRutin isaflavonoid with antioxidant, vasodilatory, anti-inflammatory and immune-stimulating activities. To study the toxicity of rutin and its protective effect, this work investigated the cytotoxic, apoptosis-inducing, genotoxic and protective effects of rutinin HTCcells. In the MTT assay, the highest concentration tested (810 mM) showed cytotoxicity after 72 h of treatment,where cell viability and cell proliferation was diminished. None of the concentration sof rutin tested induced apoptosis after 24 h treatment. The highest concentration of rutin after 24 h treatment induced DNA damage, shown in the comet assay,but did have a genotoxic effect in the micronucleus test. Rutin wastested against the pro-carcinogenic agent benzo(a)pyrene, at concentrations of 90, 270 and 810 mM, and was found to reduce induced DNA damage significantly.This protective effect of rutin against apro-carcinogen, suggests an important biological activity for this compound, whichc an contribute to human health through the diet.  aCytotoxicity  aFlavonoids  aCitogenetica  aFlavonoide  aToxidez1 aTSUBOY, M. S. F.1 aLUIZ, R. C.1 aRIBEIRO, L. R.1 aHOFFMANN-CAMPO, C. B.1 aMANTOVANI, M. S.  tExperimental and Toxicologic Pathologygv. 63, n. 5, p. 459-465, 2011.