02130naa a2200265 a 450000100080000000500110000800800410001902400350006010000230009524501310011826000090024950001160025852012220037465000170159665300200161365300330163365300110166670000210167770000230169870000220172170000170174370000240176070000210178477300590180518641692022-08-22       2010    bl uuuu     u00u1 u    #d7 a10.1007/s10681-010-0150-y2DOI1 aOLIVEIRA, E. J. de  aPolymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection.h[electronic resource]  c2010  aDisponivel em:&lthttp://www.springerlink.com/content/31625u55t2134803/fulltext.pdf.&gt Acesso em: 14 out. 2010.  aConventional methods for the selection of papaya pure lines are time-consuming. Procedures involving the use of molecular markers and the indirect selection of homozygous plants can reduce this time considerably. The objective of this study was to evaluate the informativeness of a microsatellite marker set when used in marker-assisted selection (MAS) for the development of new papaya lines. Eighty-three lines originating from two segregating F3 populations and from papaya germplasm were used for the molecular analysis of 27 microsatellite primers. Twenty polymorphic microsatellite primers were identified, allowing the identification of 86 alleles, with an average of 3.18 alleles per primer. The observed heterozygosity values were low for both the markers (0.00?0.29) and the individual lines (0.00?0.35). The inbreeding coefficient (f) ranged from 0.634 to 1.00. Eleven lines with f = 1.00 and 18 lines with f varying from 0.953 to 0.961 were identified. In addition, papaya lines showed high genetic diversity, which will certainly contribute to the evelopment of new varieties. Our results show that the use of microsatellites in MAS is a quick and effective procedure for the development of papaya lines.  ainbred lines  aMicrosatellites  aMolecular-assisted selection  aPapaya1 aSILVA, A. dos S.1 aCARVALHO, F. M. de1 aSANTOS, L. F. dos1 aCOSTA, J. L.1 aAMORIM, V. B. de O.1 aDANTAS, J. L. L.  tEuphytica, Wageningengv. 173, n. 2, p. 279-287, 2010.