01420naa a2200325 a 450000100080000000500110000800800410001902400410006010000200010124501260012126000090024752004250025665000150068165000180069665000260071465000520074065000240079265000220081665000110083865000090084965000180085865000110087670000200088770000190090770000160092670000220094270000220096470000160098677300920100218566732025-05-23       2008    bl uuuu     u00u1 u    #d7 a10.1590/S1516-891320080005000052DOI1 aSILVA, R. M. da  aDetection and identification of TMV infecting tomato under protected cultivation in Paraná State.h[electronic resource]  c2008  aDuring an inspection in plastic houses in Sapopema, Paraná, 90% of tomato plants showed leaf abnormalities, probably associated with herbicide toxity. However, virus like symptoms developed in selected hosts after mechanical inoculatation. RT-PCR reactions using primers for an internal region within the movement protein gene of TMV and ToMV resulted in the amplification of a 409 bp cDNA fragment only by TMV primers.  aGene banks  aPlasticulture  aProtected cultivation  aReverse transcriptase polymerase chain reaction  aTomato mosaic virus  aCultivo protegido  aEstufa  aGene  aPlasticultura  aTomate1 aSOUTO, E. R. de1 aPEDROSO, J. C.1 aARAKAVA, R.1 aALMEIDA, A. M. R.1 aBARBOZA, A. A. L.1 aVIDA, J. B.  tBrazilian Archives of Biology and Technologygv. 51, n. 5, p. 903-909, Sept./Oct. 2008.