01731naa a2200301 a 450000100080000000500110000800800410001910000210006024500570008126000090013852008130014765000360096065300170099665300270101365300300104065300470107065300300111765300400114765300230118765300240121070000220123470000250125670000160128170000180129770000170131570000230133277300740135510832392010-11-11 2006 bl uuuu u00u1 u #d1 aBERIAM, L. O. S. aPseudomonas syringae pv. tabaci in papaya seedlings. c2006 aThe natural ocurrence of Pseudomonas syringae pv. tabaci causing leaf spot symptoms in papaya seedlings is reported. The pathogen was identified through biochemical, physiological, serological, and molecular assays and artificial inoculations in papaya plants . It was also shown that the strains were pathogenic to bean and tobacco plants. The restriction patterns obtained with Afa I, Alu I, Dde I, Hae III, Hpa II, Hinf I, Sau 3 A I Sau 3 A I and Taq I of the PCR-RFLP of 16S-23S DNAr were identical to the P. s. pv. tabaci patterns. Primers corresponding to hrpL gene of P syringae were also tested and the results grouped the papaya strains with P s. pv. tabaci. Bacterial strains were deposited at Coleção de Culturas IBSBF, Instituto Biológico, Campinas, Brazil, under access numbers 1687 and 1822. aPseudomonas syringae pv. tabaci a16S-23S rDNA aBacteriose do mamoeiro aEnsaios de patogenicidade aLesões foliares em plântulas de mamoeiro aPapaya bacterial diseases aTestes bioquímicos e fisiológicos aTestes moleculares aTestes serológicos1 aALMEIDA, I. M. G.1 aDESTÉFANO, S. A. L.1 aGRABERT, E.1 aBALANI, D. M.1 aFERREIRA, M.1 aRODRIGUES NETO, J. tSumma Phytopatologica, Botucatugv. 32, n.1, p.21-26, Jan./Mar. 2006.