01213naa a2200193 a 450000100080000000500110000800800410001910000170006024501250007726000090020252006540021165000100086565000110087565300110088670000180089770000210091570000160093677300670095218289152011-08-18 1988 bl --- 0-- u #d1 aHAYES, R. J. aPriming of complementary DNA synthesis in vitro by small DNA molecules tightly bound to virion DNA of wheat dwarf virus. c1988 aDNA isolated from purified preparations of wheat dwarf virus (WDV) has been shown to contain tightly bound small DNA molecules which can act as primers for the synthesis of full-length complementary DNA in vitro. The small DNA molecules are bound in the terminating intergenic region of the WDV genome between the end of an open reading frame encoding a putative protein of Mr 17 292 and a conserved 'A-T' box containing putative transcriptional polyadenylation signals. Evidence that the small DNA molecules contain ribonucleotides at their 5' termini is presented and their possible role in the priming of virus DNA synthesis in vivo is discussed. aTrigo aVĂ­rus aPrimer1 aMACDONALD, H.1 aCOUTTS, R. H. A.1 aBUCK, K. W. tJournal of General Virologygv. 69, pt. 6, p. 1345-1350, 1988.