01174naa a2200145 a 450000100080000000500110000800800410001910000170006024501530007726000090023052006860023965300270092570000140095277300620096618277062013-06-18       1974    bl ---       0--  u    #d1 aMELCHERS, G.  aSomatic hybridisation of plants by fusion of protoplasts. I. Selection of light resistant hybrids of "Haploid" light sensitive varieties of tobacco.  c1974  aMesophyll protoplasts of “haploids” produced by “anther culture” of two chlorophyll deficient, light sensitive varieties of Nicotiana tabacum, sublethal (s) and virescent (v) are fused with the method of Keller and Melchers (1973). The double heterozygote hybrid of the two recessive genes for light sensitivity complements to normal leaf colour and resistance to high light intensity. By using this complementation obviously 12, possibly 21 independently arisen somatic hybrid plants are selected. Since leaf colour mutations are frequent and light sensitivity of leaf colour mutants is not too rare in higher plants, it is possible to use generally this selection method.  aFusão de protoplastos1 aLABID, G.  tMolecular and General Geneticsgv. 135, p. 277-294, 1974.