03255naa a2200277 a 450000100080000000500110000800800410001910000190006024501070007926000090018652024620019565000110265765000130266865000230268165300340270465300160273865300130275465300130276770000210278070000190280170000220282070000230284270000160286570000180288177300780289918106522003-05-29       1997    bl ---       0--  u    #d1 aQUEIROZ, A. O.  aIsoenzymatic characterization of isolates of T. evansi from the Pantanal in Mato Grosso State, Brazil.  c1997  aTrypanosoma evansi a flagellated kinetoplastid of the family Trypanosomatidae is classified within the subdiviosion Salivaria (Hoare, 1972) according to its mode of inoculation: mechanical transmission via hematophagic insects of the geni Tabanus and Stomoxys. In Latin America this parasite has acquired an additional vector, the bat Desmodus rotundus. In Brazil, T. evansi is responsible for an equine disease known as "Mal de Cadeiras", which causes ataxia in horses, thus limiting their use in raising cattle in open areas in the livestock-dependent regions of the Pantanal in Mato Grosso State and the Ilha de Marajo (Para State). We examined six isolates which were derived from the following hoists: 2 isolates from horse hosts; 2 isolates from dogs; and 2 isolates from quati (Nasua nasua, carnivora, Procyonidae) which were infected by natural means in the Pantanal of Mato Grosso State. These specimens were cryopreserved in our laboratory. Rats which had been immunosuppressed with 200mg/kg of cyclophosphamide 48 hours prior, were inocculated with the tripomastigote forms of T evansi. Blood was drawn from these inocculated rats and the parasites purified using anion-exchange chromatography on a DEAE-cellulose column (lanham and Godfrey, 1970). The purified parasites were lysed and the enzymatic activity of a certain extract was analyzed in an attempt to differentiate among the six isolates using substrates of the following enzymes: MDH [EC 1.1.1.37], G6PI [EC 5.3.1.9], G6DH [EC 1.1.1.44], IDH [EC 1.1.1.42] and ME [EC 1.1.1.40]. It was observed that all of the isolates demonstrated similar electrophoretic profiles. The homogeneity of isoenzymatic profiles of T. evansi had already been observed by other authors in isolates derived from domestic animals and capybaras (Stevens, 1989; Franke, 1994). Additionally, our results show that isolates from quati have the same profile. T. evansi has one of the widest distributions and one of the greatest range of mammalian hosts, and it is agreed that it evolved from T. brucei. This adaptability is not reflected in the variability of the biochemical parameters studied until now (although we observed significant variability in the virulence for Swiss mice). An interesting aspects of this parasite is that T. evansi was introduced in South America by domestic animals during the Spanish colonization, after which it adapted to wild animals and establieshed a sylvatic transmission cycle.  aBrazil  aPantanal  aTrypanosoma evansi  aIsoenzymatic characterization  aMato Grosso  aParasita  aParasite1 aCORREA, R. P. G.1 aMENEZES, V. T.1 aD'AVILA, A. M. R.1 aSILVA, R. A. M. S.1 aLEON, L. L.1 aJANSEN, A. M.  tMemorias do Instituto Oswaldo Cruzgv.92, p.130, Nov. 1997. Supplement 1.