02783naa a2200277 a 450000100080000000500110000800800410001910000160006024501150007626000090019152020010020065000110220165000130221265000230222565000090224865300270225765300080228465300290229265300130232165300130233470000170234770000190236470000230238370000210240677300780242718106502003-05-29       1997    bl ---       0--  u    #d1 aRAMIREZ, L.  aOccurrence of canine trypanosomiasis due to Trypanosoma evansi in the Pantanal, Brazilbhematologic disorders.  c1997  aAccording to Losos (Infectious Tropical Diseases of Domestic Animals. Essex: Longman Scientific & Technical, p.938, 1986) hematological alterations are the most consistent finding in salivarian trypanosomiasis. The pathogenesis of T. evansi and other trypanosomes of the subgenus trypanozoon is characterized by rapid loss of weight and varying degrees of anemia. Leukopenia has been reported in trypanosomosis and is attributed to reduced myelopoiesis (Jenkins, G. C. & Facer, C. A. Hematology of African Trypanosomiasis, in Tizard. I. Immunoloogy and Pathogenesis of Trypanosomiasis. Boca Raton, Florida p.13-44, 1985). In July 1997, an outbrteak of canine trypanosomiasis occurred in aranch located in Pantanal of Nhecolandia subregion. Blood samples were taken from 6 sick dogs. The dogs were bled from their radial vein using a vacuum system (Vacuum II, Labnew, Campinas, Brazil). The hematocrit (Ht) was measured using the standard method, and the red blood cell count (RBC) and total white blood cell count (WBC) were obtained using Neubauer chamber. The diagnosis of trypanosomiasis was made using the microhematocrit centrifuge test. The concentrated parasites in the buffy coat of micro hematocrit tubes were also used to, prepare thin smears. Three blood samples (50%) from 6 dogs were positive. The parasites were identified based on morphological and biometrical data. The mean hematologic values observed in infected dogs were: RBC: 1.11 x 10 mm3; WBC: 1,9 x 10 mm3; Ht: 20%. The dogs presented anemia chracterized by a low erythrocite count and decreased hematocrit as well as leukopenia. Several erythrocyte abnormalies were observd in the blood of infected dogs: microspherocytes, acanthocytes, stomatocytes, drepanocytes, dacrocytes and keratocytes. Anisocytosis and poikilocytosis were present. Adhesion of erythrocytes to trypanosome in circulating blood was also observed. We believe that several erythrocyte abnormalities contributed of to the increased red cel destruction.  aBrazil  aPantanal  aTrypanosoma evansi  aCão  aAlteracao hematologica  aDog  aHematological alteration  aParasita  aParasite1 aSOUZA, S. S.1 aBRANDAO, M. C.1 aSILVA, R. A. M. S.1 aDAVILA, A. M. R.  tMemorias do Instituto Oswaldo Cruzgv.92, p.125, Nov. 1997. Supplement 1.