03271naa a2200361 a 450000100080000000500110000800800410001910000170006024501160007726000090019352021270020265000140232965000180234365000090236165000140237065300170238465300120240165300160241365300160242965300130244565300170245870000130247570000190248870000200250770000160252770000230254370000190256670000290258570000170261470000210263170000180265277302390267018099442004-01-05       2003    bl ---       0--  u    #d1 aEGITO, A. A.  aRapd markers utilization on the formation or maintenance of conservation nuclei of different livestock species.  c2003  aGenetic characterization is an essential issue in the conservation and breeding programs of any country. In these work we report the use of RAPD markers in population genetic study of three breeds of different species that are in Brazilian genetic conservation program. The objective was to analyze the genetic similarity of individuals of those populations breeds in order to contribute to ex situ and in situ conservation. The selection of animals with least genetic similarity can help to conserve the maximum variability within a population, and can even optimize the choice of breeders for ex situ conservation programs. These studies were carried out in the Animal Genetics Laboratory (AGL) at Embrapa's Center for Genetic Resources and Biotechnology (CENARGEN). The native populations studied include: the Pantaneiro horse (in situ conservation nuclei), the bovine Crioulo Lageano (in situ conservation nuclei) and the Moxoto goat (five populations of these breed distributed in Northeast states). Using RAPD markers, obtained for each specie, we generated a Jaccard coefficient similarity matrix by NTSYS-pc v. 2.0 program. By the comparison between pairs of individuals we could chose those that were more similar between itself and those that were more distant. Using those matrix we suggested three kinds of procedure that could be used for helping the conservation of those breeds: the choice of the males to be semen donators - those less similar - avoiding duplication of samples (animals with high genetic similarity); the indication of preferential crossings aiming at the maintenance of the maximum genetic variability and the indication of animals for discarding - those that had high similarity - the breeder chose one of them for the discard. This kind of analysis could be done between two populations of the same breed in order to contribute to the genetic sample exchange between different nuclei. We must remember that these recommendations are based exclusively on genetic data and must lead in account the phenotypic characteristics of each animal, not having however to be considered separately.  alivestock  aConservação  aGado  aGenética  aConservation  aGenetic  aMaintenance  aManuntencao  aMarcador  aRapd markers1 aFUCK, B.1 aSPRITZE, A. L.1 aOLIVEIRA, R. R.1 aMcMANUS, C.1 aMARIANTE, A. da S.1 aRIBEIRO, M. N.1 aALBUQUERQUE, M. do S. M.1 aPAIVA, S. R.1 aCASTRO, S. T. R.1 aSANTOS, S. A.  tIn: CONGRESO IBEROAMERICANO DE RAZAS CRIOLLAS, 6.; SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION Y UTILIZACION DE RECURSOS ZOOGENETICOS, 4., 2003, Recife. Resumenes de Ponencias y Comunicaciones. Recife: FIRC / CYTED, 2003. nao paginado.